Optically clear nuclei in papillary carcinoma of the thyroid: Demonstration of one of the fixation artifacts and its practical usefulness
Optically clear nuclei in papillary carcinoma of the thyroid: Demonstration of one of the fixation artifacts and its practical usefulness
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DOI：
10.1046/j.1440-1827.2000.01013.x
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发表时间：
2000-02-01
影响因子：
2.2
通讯作者：
Kakudo, K
中科院分区：
文献类型：
--
作者：
Naganuma, H;Murayama, H;Kakudo, K
检查光学透明的细胞核是否是甲状腺乳头状癌的固定伪影之一。采用最佳浸没固定方法通过制作尺寸为 5 x 2 x 2 毫米的薄片样本进行实验。测试的固定剂为10%福尔马林、10%缓冲福尔马林、酒精和福尔马林的混合物（4:1）、95%酒精、4%磷酸盐缓冲多聚甲醛、Carnoy's固定剂、Bouin's固定剂和不同浓度的福尔马林。测试的甲状腺病变总共为：乳头状癌病例（38例）；滤泡性癌（1） 滤泡性腺瘤（15）；腺瘤性甲状腺肿 (11);桥本氏甲状腺炎（二）；格雷夫氏病（五）；和肿瘤周围的正常甲状腺组织 (5)，常规处理的乳头状癌显示光学透明细胞核的发生率较高 (64.6%)，而其他病变的发生率较低 (几乎为 0%)。然而，无论固定剂的选择如何，所有实验组中乳头状癌的这一比率均下降（< 35.3%）。在实验组中，在较高浓度福尔马林（40-60%）固定的乳头状癌中，经常出现（61.9-66.6%）光学透明的细胞核。这些结果表明，光学透明的细胞核是固定伪影之一，尽管作为诊断标准仍然有用。
To examine whether or not optically clear nuclei are one of the fixation artifacts in papillary carcinoma of the thyroid. Experiments using an optimal immersion fixation method by making thin-sliced specimens, 5 x 2 x 2 mm in size were adopted. Fixatives tested were 10% formalin, 10% buffered formalin, mixture of alcohol and formalin (4 : 1), 95% alcohol, 4% phosphate-buffered paraformaldehyde, Carnoy's fixative, Bouin's fixative and various concentrations of formalin, Tested thyroid lesions in total were: cases with papillary carcinoma (38); follicular carcinoma (one) follicular adenoma (15); adenomatous goiter (11); Hashimoto's thyroiditis (two); Grave's disease (five); and normal thyroid tissue around tumor (five), Routinely processed papillary carcinoma showed a high occurrence (64.6%) of the optically clear nuclei contrasted by a low occurrence (almost 0%) in other lesions. However, this rate in papillary carcinoma decreased (< 35.3%) in all experimental groups irrespective of the choice of fixatives, Frequent occurrence (61.9-66.6%) of the optically clear nuclei was reproduced in papillary carcinoma fixed in a higher concentration of formalin (40-60%) in the experimental groups. These results demonstrated that the optically clear nuclei are one of the fixation artifacts, although still useful as a diagnostic criterion.