Human papillomavirus: detection of viral DNA sequences and evidence for molecular heterogeneity in metaplasias and dysplasias of the uterine cervix.
Human papillomavirus: detection of viral DNA sequences and evidence for molecular heterogeneity in metaplasias and dysplasias of the uterine cervix.
复制标题
人乳头瘤病毒：病毒 DNA 序列的检测以及子宫颈化生和发育不良分子异质性的证据。
DOI：
10.1159/000149393
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发表时间：
1983
期刊：
影响因子：
4.6
通讯作者：
Jenson,AB
中科院分区：
文献类型：
--
作者：
Lancaster,WD;Kurman,RJ;Sanz,LE;Perry,S;Jenson,AB
本文分析了22例患者阴道镜下宫颈活检中人乳头瘤病毒（HPV） DNA和结构抗原的存在。11例显微镜下活检为轻度发育不良，3例为中度发育不良，1例为重度发育不良，7例为鳞状化生。所有标本均与牛乳头瘤病毒1型DNA探针进行非严格杂交，并与针对乳头瘤病毒属特异性结构抗原的抗血清进行免疫细胞化学分析。11例轻度发育不良患者中，5例同时检测到HPV DNA和结构抗原，3例仅检测到HPV抗原，1例仅检测到HPV DNA， 2例未检测到DNA和结构抗原。3例中度发育不良均存在HPV DNA和结构抗原。在单个严重不典型增生病例中仅检测到HPV DNA序列。在2例鳞状化生中检测到HPV DNA。其余5例宫颈活检显示鳞状化生，3例胎盘组织和6例宫颈癌，HPV DNA和结构抗原均为阴性。宫颈发育不良中HPV DNA的限制性内切酶切割模式提示有多种病毒类型或亚型与宫颈发育不良相关。与HPV 11型（HPV-11）探针的严格杂交显示，10个发育不良中只有1个含有与探针同源的序列。在其余9例发育不良中，5例含有非严格杂交检测到的HPV序列。4例鳞状化生中2例含有与HPV-11探针杂交的病毒序列，6例宫颈癌中1例含有与HPV-11探针杂交的病毒序列。
Colposcopic-directed biopsies of the uterine cervix from 22 patients were analyzed for the presence of human papillomavirus (HPV) DNA and structural antigens. 11 of the biopsies were classified microscopically as mild dysplasia, 3 as moderate dysplasia, 1 as severe dysplasia, and 7 as squamous metaplasia. Nonstringent hybridization with a bovine papillomavirus type 1 DNA probe and immunocytochemical analysis with an antiserum against papillomavirus genus-specific structural antigens were performed on all specimens. Of the 11 mild dysplasias, both HPV DNA and structural antigens were detected in 5, only HPV antigens in 3, only HPV DNA in 1, and neither DNA nor structural antigens in 2. Both HPV DNA and structural antigens were present in the 3 cases of moderate dysplasia. Only HPV DNA sequences were detected in the single case of severe dysplasia. HPV DNA was detected in 2 cases of squamous metaplasia. The 5 remaining cervical biopsies showing squamous metaplasia, tissue from 3 placentas, and 6 cervical carcinomas were negative for HPV DNA and structural antigens. Restriction enzyme cleavage patterns of HPV DNA in the dysplasias suggested that there are multiple virus types or subtypes associated with cervical dysplasia. Stringent hybridization with a HPV type 11 (HPV-11) probe revealed that only 1 of 10 dysplasias contained sequences with homology to the probe. Of the remaining 9 dysplasias, 5 contained HPV sequences detected under nonstringent hybridization. 2 of 4 squamous metaplasias contained viral sequences which hybridized to the HPV-11 probe as well as 1 of 6 cervical carcinomas.