SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
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DOI：
10.1016/0003-2697(87)90021-2
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发表时间：
1987-04-01
影响因子：
2.9
通讯作者：
SACCHI, N
中科院分区：
文献类型：
--
作者：
CHOMCZYNSKI, P;SACCHI, N
本文报道了一种用酸性硫氰酸胍-苯酚-氯仿混合液一次提取总RNA的新方法。该方法提供了高产量的未降解RNA的纯制备物，并且可以在4小时内完成。它特别适用于处理大量样品和从微量细胞或组织样品中分离RNA。
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.