Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion
Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion
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DOI：
10.1016/j.biomaterials.2010.07.108
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发表时间：
2010-12-01
期刊：
影响因子：
14
通讯作者：
Zhang, Yuanyuan
中科院分区：
文献类型：
--
作者：
Bodin, Aase;Bharadwaj, Shantaram;Zhang, Yuanyuan
本研究的目的是制备以人尿液干细胞(USC)为种子的细菌纤维素(BC)支架，以形成用于尿流改道的组织工程管道。合成微孔BC支架，诱导USC分化为尿路上皮和平滑肌细胞(SMC)。将诱导的USC(10(6)个/cm(2))在静态和三维动态(10或40转/分)条件下接种于BC上，培养2周。USC来源的尿路上皮细胞和SMC在BC支架表面形成多层膜，部分细胞渗入支架内。USC分化后的尿路上皮细胞表达尿路上皮标志物(Uroplakin 1a和AE1/AE3)，SMC表达SMC标志物(a-平滑肌肌动蛋白和结蛋白)。此外，将USC/BC支架结构植入无菌小鼠体内，用人类核抗原的免疫组织化学染色对细胞进行追踪。在体内，细胞似乎分化并表达尿路上皮和SMC标记。综上所述，多孔BC支架允许USC三维生长，导致形成多层尿路上皮和细胞基质渗透。因此，细胞接种的BC支架有望用于组织工程化尿路重建。(C)2010爱思唯尔有限公司。保留所有权利。
The objective of this study was to generate bacterial cellulose (BC) scaffolds seeded with human urine-derived stem cells (USC) to form a tissue-engineered conduit for use in urinary diversion. Microporous BC scaffolds were synthesized and USC were induced to differentiate into urothelial and smooth muscle cells (SMC). Induced USC (10(6) cells/cm(2)) were seeded onto BC under static and 3D dynamic (10 or 40 RPM) conditions and cultured for 2 weeks. The urothelial cells and SMC derived from USC formed multilayers on the BC scaffold surface, and some cells infiltrated into the scaffold. The urothelium derived from USC differentiation expressed urothelial markers (uroplakin la and AE1/AE3) and the SMC expressed SMC markers (a-smooth muscle actin and desmin). In addition, USC/BC scaffold constructs were implanted into athymic mice, and the cells were tracked using immunohistochemical staining for human nuclear antigen. In vivo, the cells appeared to differentiate and express urothelial and SMC markers. In conclusion, porous BC scaffolds allow 3 dimensional growth of USC, leading to formation of a multilayered urothelium and cell matrix infiltration. Thus, cell-seeded BC scaffolds hold promise for use in tissue-engineered urinary conduits for urinary reconstruction. (C) 2010 Elsevier Ltd. All rights reserved.