A novel type II cytokeratin, mK6irs, is expressed in the Huxley and Henle layers of the mouse inner root sheath
A novel type II cytokeratin, mK6irs, is expressed in the Huxley and Henle layers of the mouse inner root sheath
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DOI：
10.1046/j.1523-1747.2001.01226.x
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发表时间：
2001-03-01
影响因子：
6.5
通讯作者：
Ito, M
中科院分区：
文献类型：
--
作者：
Aoki, N;Sawada, S;Ito, M
毛囊分化涉及上皮型角蛋白或细胞角蛋白和毛发角蛋白以及毛发角蛋白相关蛋白的表达。在这项研究中，一个cDNA克隆编码的细胞角蛋白家族成员分离使用RNA差异显示技术。预测的氨基酸序列来源于这个克隆，揭示了同源性与许多细胞角蛋白，不仅在中央α-螺旋区，而且在保守的部分的氨基和羧基末端结构域，这表明该蛋白质代表了小鼠II型细胞角蛋白家族的一个新成员。北方印迹分析显示在小鼠皮肤中表达，但在其他组织中不表达，包括舌、食管和前胃。一维和二维蛋白质印迹分析表明，这种新的细胞角蛋白的大小为57 kDa，略低于细胞角蛋白5，这对应于细胞角蛋白6家族成员的区域。RNA原位杂交和免疫组织化学研究表明，这种细胞角蛋白的表达在生长期III和生长期VI的内根鞘的Henle和赫胥黎层的内根鞘毛锥。新的细胞角蛋白的表达开始于毛球，并进展到角化区的高度。综合分析的数据，我们将这种新型细胞角蛋白称为mK 6 irs（小鼠基因命名法K2- 6 g），以表明其在二维凝胶中与K6相似的迁移率及其在毛囊内根鞘中的特异性表达。
Hair follicle differentiation involves the expression of both epithelial-type keratins or cytokeratins and hair keratins as well as hair keratin-associated proteins. In this study, a cDNA clone encoding a cytokeratin family member was isolated using RNA differential display techniques. The predicted amino acid sequence derived from this clone, revealed a homology with a number of cytokeratins, not only in the central alpha -helical regions but also in the conserved portions of the amino and carboxy terminal domains, indicating that this protein represents a new member of the mouse type II cytokeratin family. Northern blot analysis showed expression in mouse skin, but not in other tissues, including tongue, esophagus, and forestomach. One- and two-dimensional western blot analysis showed that this new cytokeratin was 57 kDa in size and ran slightly below the area of cytokeratin 5, which corresponded to that of the cytokeratin 6 family members. Both RNA in situ hybridization and immunohistochemical studies of mouse anagen hair follicles demonstrated expression of this cytokeratin in the inner root sheath hair cone during anagen III and in the Henle and Huxley layers of the inner root sheath during anagen VI. The expression of the new cytokeratin began in the hair bulb and progressed up to the height of the keratogenous zone. Taken together the sum of the data analyzed, we have termed this novel cytokeratin mK6irs (mouse gene nomenclature k2-6g) to indicate both its similar mobility with K6 in two-dimensional gels and its specific expression in the inner root sheath of the hair follicle.