Facile Preparation of Photoactivatable Surfaces with Tuned Substrate Adhesiveness
Facile Preparation of Photoactivatable Surfaces with Tuned Substrate Adhesiveness
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DOI：
10.2116/analsci.32.1183
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发表时间：
2016-11-01
影响因子：
1.6
通讯作者：
Nakanishi, Jun
中科院分区：
文献类型：
--
作者：
Shimizu, Yoshihisa;Kamimura, Masao;Nakanishi, Jun
本文描述了一种简便的方法，用于制备具有调谐表面密度的细胞外基质肽的光活化底物，以解决生物化学和机械线索对集体细胞迁移的影响。表面配体密度的可控性通过细胞粘附和迁移试验进行验证，并辅以替代配体的荧光观察。根据表面配体密度的不同，HeLa细胞保持或失去集体特征。本材料将有助于解决集体细胞迁移的机械生物学。
This paper describes a facile method for the preparation of photoactivatable substrates with tuned surface density of an extracellular matrix peptide to resolve the impacts of biochemical and mechanical cues on collective cell migration. The controllability of surface ligand density was validated by cell adhesion and migration tests, complemented with fluorescence observation of an alternative ligand. Depending on the surface ligand density, HeLa cells either kept or lost collective characteristics. The present materials will be useful to address mechanobiology of collective cell migration.