Role of surface fimbriae (fibrils) in the adsorption of Actinomyces species to saliva-treated hydroxyapatite surfaces.
Role of surface fimbriae (fibrils) in the adsorption of Actinomyces species to saliva-treated hydroxyapatite surfaces.
复制标题
表面菌毛（原纤维）在放线菌物种吸附到唾液处理的羟基磷灰石表面中的作用。
DOI：
10.1128/iai.33.3.908-917.1981
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发表时间：
1981
影响因子：
3.1
通讯作者：
Mansheim,BJ
中科院分区：
文献类型：
--
作者：
Clark,WB;Webb,EL;Wheeler,TT;Fischlschweiger,W;Birdsell,DC;Mansheim,BJ
我们研究了选定的放线菌和相关物种的吸附、形态和血清学特征。通过电子显微镜对醋酸双氧铀染色的细胞进行评估，发现菌株之间表面菌毛频率存在很大差异。这些变化并不总是与各种放线菌菌株对唾液处理的羟基磷灰石的吸附百分比相关。然而，有 2 株 Rothia dentocarios 不具有表面菌毛，5 株 Rothia dentocarios 不具有表面菌毛。以色列的表面菌毛很少，对唾液处理的羟基磷灰石的吸附能力很弱。尽管经唾液处理的羟基磷灰石上的计算吸附位点数量在测试菌株之间差异不大，但在针对某些物种或血清型计算的亲和力中观察到显着差异。 A 菌株的平均亲和力。粘稠血清型2和A。 naeslundiiserotype 3 相似，并且这些菌株能够很好地吸附唾液处理的羟基磷灰石。对 A 的平均吸附和亲和力。内斯伦迪菌株血清型 1 和所有 A 菌株。 israeliitest 的测定值明显低于 A 测定的值。粘稠血清型 2 orA。内斯伦迪血清型 3 菌株。抗血清对 T14V 菌株的吸附抑制活性先前显示仅与免疫血清中针对 VA1 菌毛（原纤维）抗原的抗体有关，但通过用mostA预吸附抗血清而消除了该活性。粘性和 A. naelundiistrains，但不与A.以色列火车。这表明A菌株之间存在一些交叉反应性。粘性和 A.内斯伦迪但不是A. israelii.对所有A的唾液处理的羟基磷灰石的吸附。粘性和 A.测试的 naeslundiistrains 被分离自 A 的菌毛强烈抑制。粘性应变T14V。总的来说，这些数据表明某些 A 的吸附。粘性和 A. naeslundiistrains 由表面菌毛介导，其中许多表面菌毛与菌株 T14V 菌毛出现血清学交叉反应。
We studied the adsorption, morphological, and serological characteristics of selectedActinomycesand related species. Evaluation of uranyl acetate-stained cells by electron microscopy revealed wide variations among strains in the frequency of surface fimbriae. These variations did not always correlate with the percent adsorption to saliva-treated hydroxyapatite of the variousActinomycesstrains. However, two strains ofRothia dentocariosapossessing no surface fimbriae and five strains ofA. israeliipossessing very few surface fimbriae exhibited feeble adsorption to saliva-treated hydroxyapatite. Although the calculated number of adsorption sites on saliva-treated hydroxypatite did not vary widely among the strains tested, significant differences were observed in the affinities calculated for some species or serotypes. The mean affinities for strains ofA. viscosusserotype 2 andA. naeslundiiserotype 3 were similar, and these strains adsorbed well to saliva-treated hydroxyapatite. The mean adsorption and affinity for theA. naeslundiistrain serotype 1 and all strains ofA. israeliitested were significantly less than those determined for theA. viscosusserotype 2 orA. naeslundiiserotype 3 strains. Adsorption inhibition activity of antiserum to strain T14V, previously shown to be solely related to antibodies in immune serum directed against the VA1 fimbria (fibril) antigen, was removed by preadsorption of the antiserum with mostA. viscosusandA. naelundiistrains, but not withA. israeliistrains. This suggests some cross-reactivity among strains ofA. viscosusandA. naeslundiibut notA. israelii.Adsorption to saliva-treated hydroxyapatite of allA. viscosusandA. naeslundiistrains tested was strongly inhibited by fimbriae isolated fromA. viscosusstrain T14V. Collectively, these data suggest that the adsorption of certainA. viscosusandA. naeslundiistrains is mediated by surface fimbriae, many of which appear serologically cross-reactive with strain T14V fimbriae.