ACETALDEHYDE COENZYME-A DEHYDROGENASE OF ESCHERICHIA-COLI
ACETALDEHYDE COENZYME-A DEHYDROGENASE OF ESCHERICHIA-COLI
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DOI：
10.1128/jb.144.1.179-184.1980
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发表时间：
1980-01-01
影响因子：
3.2
通讯作者：
CRONAN, JE
中科院分区：
文献类型：
--
作者：
CLARK, DP;CRONAN, JE
突变体E.在有氧条件下醇脱氢酶被去抑制的大肠杆菌（adh）也过量产生乙醛CA脱氢酶。乙醛CA脱氢酶由乙醇或乙醛诱导，并受到强烈的分解代谢物阻遏，而乙醇脱氢酶受这些条件的影响很小。从adh菌株中分离出不再能够使用乙醇作为C源的突变体。这些突变体中的一些在ADH位点的回复突变体，不再产生乙醇脱氢酶或乙醛CA脱氢酶。其他的，指定acd，被发现只缺乏乙醛CA脱氢酶。acd突变位于E.大肠杆菌遗传图谱，基因顺序为thyA-lysA-acd-serA-fda。与acd共转导的Tn[转座子]10插入片段的分离大大简化了作图过程。
Mutants of E. coli (adh) in which alcohol dehydrogenase is derepressed under aerobic conditions also overproduced acetaldehyde CA dehydrogenase. Acetaldehyde CA dehydrogenase was induced by ethanol or acetaldehyde and subject to strong catabolite repression, whereas alcohol dehydrogenase was little affected by these conditions. Mutants no longer able to use ethanol as C source were isolated from an adh strain. Some of these mutants were revertants at the adh locus and no longer produced either alcohol dehydrogenase or acetaldehyde CA dehydrogenase. Others, designated acd, were found to lack only acetaldehyde CA dehydrogenase. The acd mutation was located at min 62 of the E. coli genetic map, the gene order being thyA-lysA-acd-serA-fda. Isolation of Tn[transposon]10 insertions cotransducible with acd greatly simplified the mapping procedure.