Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury.
Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury.
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DOI：
10.1371/journal.pone.0133417
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Weissleder R
中科院分区：
文献类型：
--
作者：
Chung HJ;Pellegrini KL;Chung J;Wanigasuriya K;Jayawardene I;Lee K;Lee H;Vaidya VS;Weissleder R
由于重金属或化学品等各种环境因素造成的急性和慢性肾损伤的高发病率一直是发展中国家的一个主要问题。然而，由于缺乏可应用于护理点的高度敏感和特异性技术，这些领域的肾损伤诊断更具挑战性。为了解决这个问题，我们开发了一种称为“微尿纳米颗粒检测（μUNPD）”的技术，可以检测尿液中的痕量分子标记物。具体而言，该技术利用自动化芯片上测定，然后用手持式装置进行检测以进行读出。使用μUNPD技术，检测到的肾损伤标志物KIM-1和半胱氨酸蛋白酶抑制剂C的浓度分别低至0.1 ng/ml和20 ng/ml，满足识别急性或慢性肾损伤患者所需的临界范围。因此，我们表明，μUNPD技术能够实现对肾损伤的即时和非侵入性检测，并且具有在资源有限的环境中以高灵敏度诊断肾损伤的应用潜力。
The high incidence of acute and chronic kidney injury due to various environmental factors such as heavy metals or chemicals has been a major problem in developing countries. However, the diagnosis of kidney injury in these areas can be more challenging due to the lack of highly sensitive and specific techniques that can be applied in point-of-care settings. To address this, we have developed a technique called ‘micro-urine nanoparticle detection (μUNPD)’, that allows the detection of trace amounts of molecular markers in urine. Specifically, this technique utilizes an automated on-chip assay followed by detection with a hand-held device for the read-out. Using the μUNPD technology, the kidney injury markers KIM-1 and Cystatin C were detected down to concentrations of 0.1 ng/ml and 20 ng/ml respectively, which meets the cut-off range required to identify patients with acute or chronic kidney injury. Thus, we show that the μUNPD technology enables point of care and non-invasive detection of kidney injury, and has potential for applications in diagnosing kidney injury with high sensitivity in resource-limited settings.