Viral messenger RNA unmethylated in the 5'-terminal guanosine in interferon-treated HeLa cells infected with vesicular stomatitis virus.
Viral messenger RNA unmethylated in the 5'-terminal guanosine in interferon-treated HeLa cells infected with vesicular stomatitis virus.
复制标题
在经干扰素处理的感染水泡性口炎病毒的 HeLa 细胞中，病毒信使 RNA 5 端鸟苷未甲基化。
DOI：
10.1016/0042-6822(81)90290-7
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Baglioni,C
中科院分区：
文献类型：
--
作者：
deFerra,F;Baglioni,C
研究了干扰素处理的水疱性口炎病毒（VSV）感染细胞中病毒mRNA在多体和非多体部分的分布。这些细胞合成的病毒mRNA有一半以上在非多体部分，而对照细胞合成的mRNA只有不到三分之一在非多体部分。多体和非多体mRNA在蔗糖密度梯度上沉积相同，但来自干扰素处理细胞的非多体mRNA处于低甲基化状态，用[甲基- 3h]蛋氨酸标记实验表明。核酸酶消化后对VSV mRNA的分析显示，所有分子都有帽化，但从干扰素处理细胞中分离的非多体mRNA中约有60%在5 '端g未甲基化。当在蛋白质合成起始试验中进行测试时，只有45%的mRNA与核糖体结合，相比之下，来自对照或干扰素处理细胞的多体mRNA结合率为80%。
The distribution of viral mRNA between polysomal and nonpolysomal fractions was investigated in interferon-treated cells infected with vesicular stomatitis virus (VSV). More than half of the viral mRNA synthesized by these cells is in the nonpolysomal fraction, whereas less than one-third of the mRNA synthesized by control cells is found in this fraction. Polysomal and nonpolysomal mRNA sediment identically on sucrose density gradients, but the nonpolysomal mRNA from interferon-treated cells in under-methylated, as shown by labeling experiments with [methyl-3H]methionine. Analysis of VSV mRNA after digestion with nucleases shows that all molecules are capped but about 60% of the nonpolysomal mRNA isolated from interferon-treated cells is unmethylated in the 5′-terminal G. When tested in an assay for initiation of protein synthesis, only 45% of this mRNA binds to ribosomes, as compared to 80% binding for polysomal mRNA from either control or interferon-treated cells.