LIMB DEFORMITY PROTEINS DURING AVIAN NEURULATION AND SENSE ORGAN DEVELOPMENT
LIMB DEFORMITY PROTEINS DURING AVIAN NEURULATION AND SENSE ORGAN DEVELOPMENT
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DOI：
10.1002/aja.1002040206
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发表时间：
1995-10-01
影响因子：
2.5
通讯作者：
ZELLER, R
中科院分区：
文献类型：
--
作者：
DELAPOMPA, JL;JAMES, D;ZELLER, R
核肢畸形 (Ld) 蛋白 (formins) 在鸟类原条阶段表达。最初，它们主要在形成脊索、分散的中胚层前体和诱导神经板的细胞中检测到。在内胚层细胞中未检测到表达。随后 Ld 阳性细胞沿神经管前后轴的分级分布遵循其分化的前后进展。 Ld 蛋白在内耳和眼睛的诱导和发育过程中也有差异表达。在其初始模式期间观察到 Ld 蛋白沿耳囊背腹轴的不均匀分布。在眼睛中，Ld 蛋白在晶状体基板的二次诱导过程中由视泡表达。诱导后，这些蛋白质也由新形成的晶状体基板表达，这一过程让人想起同源诱导。在视网膜和晶状体分化过程中，Ld 域似乎划分了区域，从而产生了特定的眼睛结构。对两个感觉器官中 Ld 分布和 BrdU 掺入的比较分析表明，这些蛋白质主要由定型和/或分化（有丝分裂后）细胞表达。一般来说，Ld 蛋白的表达在测定过程中被诱导，并在特定细胞类型的分化过程中保持不变。这项研究表明核 Ld 蛋白参与神经外胚层和中胚层结构的形态发生。 (C) 1995 Wiley-Liss, Inc.
The nuclear Limb deformity (Ld) proteins (formins) are expressed during the avian primitive streak stages. Initially, they are detected predominantly in cells of the forming notochord, scattered mesodermal precursors and the induced neural plate. No expression is detected in endodermal cells. The subsequent graded distribution of Ld positive cells along the anterior-posterior axis of the neural tube follows the antero-posterior progression of its differentiation. The Ld proteins are also differentially expressed during induction and development of both the inner ear and eye. An unequal distribution of Ld proteins along the dorso-ventral axis of the otic vesicle is observed during its initial patterning. In the eye, the Ld proteins are expressed by the optic vesicle during secondary induction of the lens placode. Following induction, the proteins are also expressed by the newly formed lens placode, a process which is reminiscent of homeogenetic induction. During differentiation of the retina and lens, the Ld domains seem to demarcate territories, giving rise to specific eye structures. A comparative analysis of the Ld distribution and BrdU incorporation in the two sense organs indicates that the proteins are predominantly expressed by committed and/or differentiating (post-mitotic) cells. In general, expression of Ld proteins is induced during determination and remains during differentiation of particular cell-types. This study implies that the nuclear Ld proteins are involved in morphogenesis of both neuro-ectodermal and mesodermal structures. (C) 1995 Wiley-Liss, Inc.