Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells
Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells
复制标题
DOI：
10.1002/smll.201101193
复制
发表时间：
2011-11-04
期刊：
影响因子：
13.3
通讯作者：
Bertrand, Jean-Remi
中科院分区：
文献类型：
--
作者：
Alhaddad, Anna;Adam, Marie-Pierre;Bertrand, Jean-Remi
研究了金刚石纳米颗粒（纳米金刚石，ND）将小干扰RNA（siRNA）递送到尤文肉瘤细胞中的能力，以期实现体内抗癌核酸药物递送的可能性。siRNA被吸附到用阳离子聚合物包被的ND上。通过利用来自嵌入的色心缺陷的ND的固有荧光来证明ND的细胞摄取。这些涂覆的ND的细胞毒性显示为低。与内化功效一致，在含血清培养基中通过ND载体化的siRNA在mRNA和蛋白质水平显示出对EWS/Fli-1基因表达的特异性抑制。
The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium.