Polymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome
Polymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome
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DOI：
10.1016/j.ajog.2008.10.039
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发表时间：
2009-03-01
影响因子：
9.8
通讯作者：
Witkin, Steven S.
中科院分区：
文献类型：
--
作者：
Lev-Sagie, Ahinoam;Prus, Diana;Witkin, Steven S.
目的：检测外阴前庭炎综合征（VVS）患者和对照组炎症小体NALP3成分基因编码多态性，炎症小体是调节白细胞介素(IL)-1 β产生的细胞质结构。研究设计：对143名VVS女性和182名对照女性的DNA进行了编码NALP3基因的内含子4 （CIAS1）长度多态性检测。检测前庭组织中NALP3的表达。全血培养检测白色念珠菌诱导IL-1 β的产生。结果：对照组12等位基因频率高于VVS患者（P = 0.02）。在VVS和自述复发性外阴阴道念珠菌病（RVVC）病史的患者中，等位基因7的频率为43.9%，而无RVVC病史的患者为30.8%，对照组为26.9% （P = 0.035）。001和对照受试者)。在前庭组织中检测到NALP3。在具有7,7基因型的女性样本中，白色念珠菌诱导的IL-1 β产生减少（P = 0.030）。结论：CIAS1基因多态性可能在部分患者的VVS触发中起核心作用。
OBJECTIVE: Patients with vulvar vestibulitis syndrome (VVS) and control subjects were tested for a polymorphism in the gene coding for the NALP3 component of inflammasomes, cytoplasmic structures regulating interleukin (IL)-1 beta production.STUDY DESIGN: DNA from 143 women with VVS and 182 control women were tested for a length polymorphism in intron 4 of the gene (CIAS1) that codes for NALP3. Vestibular tissue was examined for NALP3 expression. Whole blood cultures were tested for Candida albicans-induced IL-1 beta production.RESULTS: The allele 12 frequency was higher in control subjects than in the patients with VVS (P = .02). Among patients with VVS and a self-reported history of recurrent vulvovaginal candidiasis (RVVC), the allele 7 frequency was 43.9% as compared with 30.8% in patients with no history of RVVC and 26.9% in control women (P = .035 vs other patients and .001 vs control subjects). NALP3 was identified in vestibular tissue. C albicans-induced IL-1 beta production was reduced in samples from women with the 7,7 genotype (P = .030).CONCLUSION: Polymorphism in the CIAS1 gene may play a central role in the triggering of VVS in a subset of patients.