Characterization and sources of dissolved and particulate phosphorus in 10 freshwater lakes with different trophic statuses in China by solution P-31 nuclear magnetic resonance spectroscopy
Characterization and sources of dissolved and particulate phosphorus in 10 freshwater lakes with different trophic statuses in China by solution P-31 nuclear magnetic resonance spectroscopy
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溶液P-31核磁共振波谱分析中国10个不同营养状态淡水湖泊溶解磷和颗粒磷的表征及来源
DOI：
10.1111/1440-1703.1006
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发表时间：
2019
影响因子：
2
通讯作者：
Sun Fuhong
中科院分区：
文献类型：
--
作者：
Zhang Chen;Feng Weiying;Chen Haiyan;Zhu Yuanrong;Wu Fengchang;Giesy John P.;He Zhongqi;Wang Hao;Sun Fuhong
溶解性磷(DP)和颗粒态磷(PP)的信息对于评价磷的动态变化和控制富营养化具有重要意义。本文用31P溶液核磁共振波谱分析了10个淡水湖不同营养状态下的DP和PP。结果表明，DP和PP的主要形态为正磷酸盐(Ortho-P)和单酯磷酸(Mono-P)。中富营养化湖泊水体和颗粒物中正磷和单磷的浓度高于轻度富营养化湖泊和中富营养化湖泊。水和颗粒物中单磷的主要化学形态为α-甘油磷酸(2.7-32.5%)、β-甘油磷酸(1.3-23.4%)、鸟苷2‘-单磷(20.2-29.3%)、六磷酸肌醇(8.3-36.4%)和烟酰胺腺嘌呤二核苷酸(17.3-35.9%)，主要来源于不稳定的双酯、水生/微生物来源和陆源和水生/微生物来源的降解。磷酸二酯(Di-P)主要由磷壁酸(0.5-14.8%)和脱氧核糖核酸(0.6-17.7%)组成，它们来源于水生/微生物来源。相关分析表明，水体和颗粒物中的溶解态单磷与叶绿素a(Chl-a)呈正相关，表明它们对藻类活动具有潜在的生物有效性。PH是控制水中Di-P、焦磷酸盐(Pyro-P)和聚磷酸盐(Poly-P)的关键参数。单P/PPS与Chl-a(R2= 0.459)、总磷(TP)(R2= 0.586)和营养状况指数(R2= 0.588)呈正相关，表明颗粒态单P对湖泊富营养化有潜在的贡献。正磷和单磷都是藻类磷营养的主要贡献者，因此需要源头控制和新的技术来减少富营养化。
Information on dissolved phosphorus (DP) and particulate phosphorus (PP) is essential to evaluate the P dynamics and control eutrophication. In this work, DP and PP in 10 freshwater lakes representing various trophic statuses were analyzed by solution31P nuclear magnetic resonance spectroscopy. The results indicated that the predominant forms of DP and PP were orthophosphate (Ortho‐P) and monoester phosphate (Mono‐P). There was a greater concentration of Ortho‐P and Mono‐P in the water and particulate matter of medium‐eutriphic lakes than in lightly eutriphic or mesotrophic lakes. α‐Glycerophophate (2.7–32.5%), β‐glycerophosphate (1.3–23.4%), guanosine 2′Mono‐P (20.2–29.3%), inositol hexakisphosphate (IHP) (8.3–36.4%) and nicotinamide adenine dinucleotide (NAD) (17.3–35.9%) were identified as the major chemical forms of Mono‐P in water and particulate matter, which originate mainly from the degradation of labile diesters, aquatic/microbial sources and a combination of terrestrial and aquatic/microbial sources. Diester phosphate (Di‐P) was dominated by teichoic acid (0.5–14.8%) and deoxyribonucleic acid (DNA) (0.6–17.7%), which originated from aquatic/microbial sources. Moreover, correlation analysis showed that dissolved Mono‐P in water and particulate matter had a positive correlation with chlorophyll‐a(Chl‐a), which indicated their potential bioavailability for algal activity. pH was a crucial parameter to control Di‐P, pyrophosphate (Pyro‐P) and polyphosphate (Poly‐P) in water. Mono‐P/PPs showed a positive correlation with Chl‐a(R2= 0.459), total phosphorus (TP) (R2= 0.586) and the trophic status index (R2= 0.588), which suggested that particulate Mono‐P can potentially contribute to lake eutrophication. Both Ortho‐P and Mono‐P were major contributors of P nutrients for algae, and therefore source control and new techniques are needed for reducing eutrophication.