The BnTFL1–BnGF14nu–BnFD module regulates flower development and plant architecture in Brassica napus
The BnTFL1–BnGF14nu–BnFD module regulates flower development and plant architecture in Brassica napus
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DOI：
10.1016/j.cj.2023.10.002
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发表时间：
2023-10
期刊：
影响因子：
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通讯作者：
Jianjun Wang;Chi Zhang;Youpeng Chen;Yanan Shao;Meifang Liao;Qian Hou;Weitang Zhang;Yang Zhu;Yuan Guo;Zijin Liu;Christian Jung;Mingxun Chen
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文献类型：
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作者：
Jianjun Wang;Chi Zhang;Youpeng Chen;Yanan Shao;Meifang Liao;Qian Hou;Weitang Zhang;Yang Zhu;Yuan Guo;Zijin Liu;Christian Jung;Mingxun Chen
甘蓝型油菜的花发育和植株构型决定了机械化采收的效率和种子产量。虽然TERMINAL FLOW 1(AtTFL1)是拟南芥中花发育的调节基因，但TFL1的功能和调控机制还不清楚。Napus仍不清楚。基因组中的SixBnTFL1类同源基因。甘蓝型油菜自交系‘K407’在春化过程中表达水平稳步上升。CRISPR/Cas诱变最多4个BnTFL1亲本可导致早花和植株构型的改变，而BnTFL1单突变体、双突变体和三突变体的种子产量没有改变。SixBnTFL1对开花时间、总花数、顶花数和植株构型的调控作用是相加的和保守的，而BnaA02.TFL1不是。BnaA10.TFL1通过BnaA05.GF14nu蛋白与BnaA08.FD相互作用，调控花的发育，导致花整合子和花分生组织识别基因的转录抑制。这些关于控制花发育和植物结构的调控网络的发现为改变INB的这些特征提供了一条很有希望的途径。油菜籽。
Flower development and plant architecture determine the efficiency of mechanized harvest and seed yield inBrassica napus. AlthoughTERMINAL FLOWER 1(AtTFL1) is a regulator of flower development inArabidopsis thaliana, the function and regulatory mechanism ofTFL1orthologs inB. napusremains unclear. SixBnTFL1paralogs in the genome of theB. napusinbred line ‘K407’ showed steadily increasing expression during vernalization. CRISPR/Cas-induced mutagenesis of up to fourBnTFL1paralogs resulted in early flowering and alteration of plant architecture, whereas seed yield was not altered inBnTFL1single, double, or triple mutants. SixBnTFL1paralogs, but notBnaA02.TFL1, showed an additive and conserved effect on regulating flowering time, total and terminal flower number, and plant architecture. BnaA10.TFL1 regulates flower development by interacting with BnaA08.FD through the protein BnaA05.GF14nu, resulting in the transcriptional repression of floral integrator and floral meristem identity genes. These findings about the regulatory network controlling flower development and plant architecture present a promising route to modifying these traits inB. napus.