EXPOSURE OF TRYPTOPHANYL RESIDUES IN PROTEINS - QUANTITATIVE-DETERMINATION BY FLUORESCENCE QUENCHING STUDIES
EXPOSURE OF TRYPTOPHANYL RESIDUES IN PROTEINS - QUANTITATIVE-DETERMINATION BY FLUORESCENCE QUENCHING STUDIES
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DOI：
10.1021/bi00648a035
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发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
GHIRON, CA
中科院分区：
文献类型：
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作者：
EFTINK, MR;GHIRON, CA
M. R. Eftink和C. A. Ghiron*摘要：丙烯酰胺是一种有效的色氨酸-苯基荧光猝灭剂，我们报告在感知蛋白质中这种残留物的暴露程度时非常有区别。猝灭反应涉及猝灭剂和激发的吲哚环之间的物理接触，并且可以根据碰撞和静态分量进行动力学描述。碰撞组分的速率常数是蛋白质中残基暴露的动力学度量，其值范围从充分暴露于促肾上腺皮质激素多肽中的色氨酸的4 × 109 M-1 s~*到未暴露于azurin中的残基的< 5 × 108 M-1 s~ 1
M. R. Eftink and C. A. Ghiron* abstract: Acrylamide is an efficient quencher of trypto-phanyl fluorescence which we report to be very discriminat-ing in sensing the degree of exposure of this residue in pro-teins. The quenching reaction involves physical contact be-tween the quencher and an excited indole ring, and can be kinetically described in terms of a collisional and a static component. The rate constant for the collisional component is a kinetic measure of the exposure of a residue in a pro-tein, and values ranging from 4 X 109 M-1 s~* for the fully exposed tryptophan in the polypeptide, adrenocorticotropin, to< 5 X 108 M-1 s-1 for the buried residue in azurin have