Expression of Serum miR-16, let-7f, and miR-21 in Patients with Hepatocellular Carcinoma and their Clinical Significances
Expression of Serum miR-16, let-7f, and miR-21 in Patients with Hepatocellular Carcinoma and their Clinical Significances
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DOI：
10.7754/clin.lab.2013.130133
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发表时间：
2014-01-01
影响因子：
0.7
通讯作者：
Ding, Yi-Tao
中科院分区：
文献类型：
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作者：
Ge, Wei;Yu, De-Cai;Ding, Yi-Tao
背景：近年来的研究表明，血清microRNA在包括肝细胞癌（HCC）在内的多种肿瘤中异常表达。在我们以前的研究中，已经筛选了HCC中的血清microRNA（miRNA）谱。方法：选取90例HCC患者和60例非HCC患者作为研究对象，60例正常人作为对照组。采用实时逆转录聚合酶链反应测定血清microRNA的表达。结果：血清miR-16、let-7 f和miR-21在HCC中表达下调，而miR-98和miR-221在HCC中表达不明显。此外，这些miRNAs具有特定的临床意义。血清miR-16的表达在肿瘤直径大于5 cm的患者中下调（p = 0.0013），并且与一些定量临床特征如血小板（p = 0.0255）、PT（p = 0.0007）和胆红素（p = 0.0025）相关。肿瘤直径大于5cm（p = 0.0367）和早期复发（p = 0.0047）的患者血清let-7 f表达上调。结论：血清miR-16、let-7 f和miR-21与HCC的生物学行为有关，可作为估计HCC肿瘤大小或复发的潜在指标。
Background: Recent studies showed that serum microRNAs were aberrantly expressed in cancers, including hepatocellular carcinoma (HCC). Serum microRNA (miRNA) profiles in HCC have been filtered in our previous studies. In this research, some serum miRNAs were further validated and their clinical significances were analyzed.Methods: Sera from 90 HCC patients, the subjects, and 60 non-HCC normal, the controls, were used. The expression of serum microRNAs was measured using real-time reverse transcription-polymerase chain reaction. AFP, as well as other clinical materials, were summarized from the HCC databases in our hospital.Results: Serum miR-16, let-7f, and miR-21 are down-regulated in HCC while miR-98 and miR-221 are not regulated significantly. Moreover, these miRNAs are of specific clinical significance. The expression of serum miR-16 is down-regulated in the patients with a tumor more than 5 cm in diameter (p = 0.0013) and is correlated with some quantitative clinical features such as platelets (p = 0.0255), PT (p = 0.0007) and bilirubin (p = 0.0025). The expression of serum let-7f is up-regulated in the patients with a tumor more than 5 cm in diameter (p = 0.0367) and early recurrence (p = 0.0047). The expression of serum miR-21 is up-regulated in female patients with HCC (p = 0.0297).Conclusions: Serum miR-16, let-7f, and miR-21 are related with the biological behavior of HCC, which means that they could be considered as the potential indicators to estimate the tumor size or the recurrence of HCC.