Oxygen levels rapidly modulate Pseudomonas aeruginosa social behaviours via substrate limitation of PqsH.
Oxygen levels rapidly modulate Pseudomonas aeruginosa social behaviours via substrate limitation of PqsH.
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DOI：
10.1111/j.1365-2958.2010.07303.x
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发表时间：
2010-09
影响因子：
3.6
通讯作者：
Whiteley M
中科院分区：
文献类型：
--
作者：
Schertzer JW;Brown SA;Whiteley M
Many bacteria use extracellular signals to coordinate group behaviors, a process referred to as quorum sensing (QS). The bacterium Pseudomonas aeruginosa utilizes a complex QS system to control expression of over 300 genes, including many involved in host colonization and disease. The Pseudomonas Quinolone Signal (PQS) is a component of P. aeruginosa QS, and although it contributes to virulence in some models of infection, the PQS biosynthetic pathway is not fully elucidated. Here, we show that PqsH catalyzes the terminal step in PQS production, synthesizing PQS in vitro using the substrates 2-heptyl-4-quinolone (HHQ), NADH, and oxygen. Structure function studies reveal that the alkyl side chain of HHQ is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons. Due to the PqsH requirement for oxygen, PQS and PQS-controlled virulence factors are not produced by anaerobic P. aeruginosa. Interestingly, anaerobic P. aeruginosa produced PQS in the absence of de novo protein synthesis upon introduction of oxygen, indicating that oxygen is the sole limiting substrate during anaerobic growth. We propose a model in which PqsH poises anaerobic P. aeruginosa to activate PQS-controlled factors immediately upon exposure to molecular oxygen.
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影响因子：
--
作者：
Diggle, Stephen P.;Matthijs, Sandra;Williams, Paul
通讯作者：
Williams, Paul
影响因子：
3.2
作者：
Coleman, James P.;Hudson, L. Lynn;Pesci, Everett C.
通讯作者：
Pesci, Everett C.
基因组分析表明，铜绿假单胞菌的毒力是组合。
影响因子：
12.3
作者：
Lee DG;Urbach JM;Wu G;Liberati NT;Feinbaum RL;Miyata S;Diggins LT;He J;Saucier M;Déziel E;Friedman L;Li L;Grills G;Montgomery K;Kucherlapati R;Rahme LG;Ausubel FM
通讯作者：
Ausubel FM
影响因子：
3.2
作者：
Farrow, John M., III;Pesci, Everett C.
通讯作者：
Pesci, Everett C.
影响因子：
3.1
作者：
Filiatrault, MJ;Wagner, VE;Passador, L
通讯作者：
Passador, L