Serotypic characterization of outer capsid spike protein VP4 of vervet monkey rotavirus SA11 strain
Serotypic characterization of outer capsid spike protein VP4 of vervet monkey rotavirus SA11 strain
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DOI：
10.1007/s007050050371
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发表时间：
1998-01-01
影响因子：
2.7
通讯作者：
Kapikian, AZ
中科院分区：
文献类型：
--
作者：
Hoshino, Y;Jones, RW;Kapikian, AZ
黑长尾猴轮状病毒SA 11是A组轮状病毒的原型株，已显示具有VP 7血清型3特异性，但其关于其它外部衣壳蛋白VP 4的中和特异性尚未阐明。因此，我们通过与豚鼠抗血清的双向交叉中和来确定其VP 4特异性，所述抗血清是用仅具有来自猴轮状病毒SA 11株的VP 4编码基因和来自人轮状病毒DS-1株（G血清型2）的剩余10个基因的单基因置换抑制剂制备的。SA 11 VP 4与恒河猴轮状病毒MMU 18006 VP 4（P5 B毒株）在抗原性上呈单向相关，与人和犬轮状病毒VP 4（P血清型5A特异性）的抗原性略相关。此外，SA 11 VP 4显示出与其他已知P血清型（1-4和6-11）以及未表征的马、兔和禽轮状病毒株的抗原性不同。因此，建议将SA 11 VP 4分类为P5 B血清型。
The vervet monkey rotavirus SA11, a prototype strain of group A rotaviruses, has been shown to possess VP7 serotype 3 specificity but its neutralization specificity with regard to the other outer capsid protein VP4 has not been elucidated. We thus determined its VP4 specificity by two-way cross-neutralization with guinea pig antiserum prepared with a single gene substitution reassortant that had only the VP4-encoding gene from the simian rotavirus SA11 strain and remaining ten genes from human rotavirus DS-1 strain (G serotype 2). The SA11 VP4 was related antigenically in a one-way fashion to rhesus monkey rotavirus MMU18006 VP4 (a P5B strain) and marginally to human and canine rotavirus VP4s with P serotype 5A specificity. In addition, the SA11 VP4 was shown to be distinct antigenically from those of other known P serotypes (1-4, and 6-11) as well as those of uncharacterized equine, lapine, and avian rotavirus strains. The SA11 VP4 is thus proposed for classification as a P5B serotype.