Changes in Cell Membrane and Cellular Lipids in Apoptotic Cells Induced by Dolichyl Phosphate Differ from Findings in Cells Induced by Etoposide
Changes in Cell Membrane and Cellular Lipids in Apoptotic Cells Induced by Dolichyl Phosphate Differ from Findings in Cells Induced by Etoposide
复制标题
磷酸多萜酯诱导的凋亡细胞中细胞膜和细胞脂质的变化与依托泊苷诱导的细胞的变化不同
DOI：
10.5650/jos.51.35
复制
发表时间：
2002
影响因子：
1.5
通讯作者：
M. Oshima
中科院分区：
文献类型：
--
作者：
Akiko Horiuchi;E. Yasugi;C. Iwasaki;K. Fujimoto;M. Oshima
比较了磷酸多酚酯（dolichyl phosphate，dol-P）和足叶乙甙（etoposide）对U937细胞凋亡过程中细胞膜流动性和细胞脂质的影响。DOL-P诱导细胞膜扰动后立即加入，并持续至少40分钟。然而，依托泊苷诱导细胞凋亡，而不增加细胞膜流动性。在dol-P和依托泊苷处理的细胞中，处理4小时后，磷脂酰乙醇胺与磷脂酰丝氨酸一起易位。在dol-P诱导的细胞中，脂质水平的浓度发生变化，胆固醇/磷脂比几乎翻了一番。相反，依托泊苷处理的细胞中磷脂和胆固醇水平无变化。虽然dol-P和依托泊苷诱导细胞凋亡，dol-P，而不是依托泊苷，出现作为膜流动性的调节剂和改变膜成分和膜生理过程中的细胞凋亡。
Cell membrane fluidity and changes of cellular lipids of U937 cells during apoptosis are compared between the cells treated with dolichyl phosphate (dol-P) and etoposide. Dol-P induced lateral cell membrane perturbation immediately after addition and continued for at least 40 min. However, etoposide induced apoptosis without increase in cell membrane fluidity. Phosphatidylethanolamine was translocated together with phosphatidylserine after 4 hr of treatment in both dol-P and etoposide treated cells. The concentration of lipid levels changed in dol-P-induced cells and the cholesterol/phospholipid ratio almost doubled. Conversely, none of the phospholipid and cholesterol levels changed in etoposide treated cells. Although both dol-P and etoposide induce apoptosis, dol-P, but not etoposide, appeared to function as a modulator of membrane fluidity and to change membrane constituents and membrane physiology during the course of apoptosis.
影响因子：
11.2
作者：
M. Barry;J. Reynolds;A. Eastman
通讯作者：
M. Barry;J. Reynolds;A. Eastman