IMMUNE MODIFICATION OF THE PATHOGENESIS OF STREPTOCOCCUS-UBERIS MASTITIS IN THE DAIRY-COW
IMMUNE MODIFICATION OF THE PATHOGENESIS OF STREPTOCOCCUS-UBERIS MASTITIS IN THE DAIRY-COW
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DOI：
10.1111/j.1574-695x.1994.tb00432.x
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发表时间：
1994-02-01
影响因子：
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通讯作者：
LEIGH, JA
中科院分区：
文献类型：
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作者：
HILL, AW;FINCH, JM;LEIGH, JA
在奶牛停止哺乳前14天和产犊前14天，每组4头奶牛皮下接种Uberis活链球菌株0140J或从同一株链球菌表面提取的提取物。两组都在干燥后7天接受了表面提取物的乳房内给药。第三组未接种疫苗的动物作为对照。在用疫苗株对每头奶牛进行两个季度的乳房内攻击后，对照组和只接种表面提取物的奶牛的所有季度都发生了临床性乳房炎。然而，在接种了活细菌疫苗的奶牛的挑战宿舍中，只有12.5%的奶牛发生了临床性乳房炎。此外，与接种活疫苗的奶牛相比，对照和提取疫苗接种的奶牛在攻击后牛奶中的细菌数量高出10(5)倍。与提取疫苗组或对照组相比，接种活生物体的动物血清中Uberis特异性Ig G水平升高，而在整个实验过程中，所有组中Su-uberis特异性Ig G(1)和Ig M水平相似。牛奶中的特异性抗体水平不受疫苗接种的影响。尽管免疫球蛋白水平升高，但在任何血清或牛奶样本中都没有检测到调理活性的增加。活体免疫动物的外周血淋巴细胞在体外对Uberis抗原的增殖反应显著高于对照和提取物免疫动物的淋巴细胞。这些结果表明，中性粒细胞和特异性调理抗体可能不构成对Uberis感染的主要防御。
Two groups of 4 cows were vaccinated subcutaneously with live Streptococcus uberis strain 0140J or a surface extract derived from the same strain, at 14 days prior to the cessation of lactation (drying off) and at calving. Both groups also received an intramammary administration of the surface extract 7 days after drying off. A third group of unvaccinated animals acted as controls. Following intramammary challenge of two quarters per cow with the vaccine strain, all quarters on control cows and those vaccinated only with surface extract developed clinical mastitis. However, only 12.5% of challenged quarters on cows which were vaccinated with live bacteria developed clinical mastitis. In addition, the numbers of bacteria in the milk following challenge were 10(5) times higher from the control and extract vaccinated cows than those which received live vaccine. Serum levels of S. uberis specific IgG, were elevated in the animals vaccinated with the live organism when compared to that of either extract-vaccinates or controls, whilst S. uberis specific levels of IgG(1) and IgM were similar in all groups throughout the experiment. Specific antibody levels in milk were unaffected by vaccination. Despite increased levels of IgG(2), no increase in opsonic activity was detected in any serum or milk samples. Peripheral blood lymphocytes from animals vaccinated with live organisms showed a considerable increase in proliferative response to S. uberis antigen in vitro when compared with lymphocytes from control and extract-vaccinated animals. These results suggest that neutrophils and specific opsonising antibody may not form the major defence against infection with S. uberis.