Study of antibodies against human melanoma produced by somatic cell hybrids.
Study of antibodies against human melanoma produced by somatic cell hybrids.
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研究体细胞杂交产生的抗人黑色素瘤抗体。
DOI：
10.1073/pnas.75.7.3405
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发表时间：
1978
影响因子：
11.1
通讯作者：
M. Herlyn
中科院分区：
文献类型：
--
作者：
H. Koprowski;Z. Steplewski;D. Herlyn;M. Herlyn
我们融合脾淋巴细胞获得免疫小鼠对人黑色素瘤细胞系和黑色素瘤-小鼠杂交细胞与P3 X 63 Ag 8小鼠骨髓瘤，以产生杂交分泌抗体对人黑色素瘤。单个杂交瘤分泌的抗体进行了测试，他们的反应与一组人黑色素瘤，结直肠癌，和正常细胞的间接放射免疫测定，他们表现出不同的特异性和交叉反应性。一些只与黑色素瘤反应，而另一些则与正常人或人结肠直肠癌细胞交叉反应。通过单克隆抗体混合物的竞争结合分析，有可能描绘黑色素瘤上的不同表位。在裸鼠体内生长的杂交瘤产生的抗黑色素瘤抗体抑制了黑色素瘤的生长。
We fused spleen lymphocytes obtained from mice immunized against a human melanoma cell line and melanoma-mouse hybrid cells with the P3 X 63 Ag8 mouse myeloma in order to produce hybrids secreting antibodies against a human melanoma. Antibodies secreted by individual hybrids were tested for their reaction with a panel of human melanoma, colorectal carcinoma, and normal cells in an indirect radioimmunoassay, and they displayed different specificities and crossreactivities. Some reacted only with melanomas, whereas others crossreacted with normal human or human colorectal carcinoma cells. By analysis of competitive binding of mixtures of monoclonal antibodies, it was possible to delineate different epitopes on melanomas. Hybrids growing in nude mice and producing antimelanoma antibody suppressed growth of melanoma tumors.