Duration Control of Protein Expression in Vivo by Light-Mediated Reversible Activation of Translation
Duration Control of Protein Expression in Vivo by Light-Mediated Reversible Activation of Translation
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DOI：
10.1021/acschembio.6b00684
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发表时间：
2017-02-01
影响因子：
4
通讯作者：
Ogasawara, Shinzi
中科院分区：
文献类型：
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作者：
Ogasawara, Shinzi
蛋白质表达的光控制使得能够在活细胞或生物体中时空诱导生物事件。然而，常用的方法如光可控转录因子或笼状核酸。不适于精确控制蛋白质表达的持续时间。在这里，我报告的光可控帽（PC帽），可以控制mRNA的翻译在一个可逆的方式通过其顺反光异构化，通过370和430 nm的光照射。反式形式的2-间甲基-苯基偶氮帽（mMe-2 PA-cap）使斑马鱼胚胎中的翻译沉默，而与反式形式相比，用顺式形式处理提供了7.1倍的翻译蛋白量。此外，通过用370 nm光照射胚胎激活的翻译通过随后用430 nm光照射再次快速失活。通过控制斜视蛋白的表达周期，光诱导双头斑马鱼的发育，证明了这种方法的应用。
The photocontrol of protein expression enables the spatiotemporal induction of biological events in living cells or organisms. However, commonly used method such as photocontrollable transcription factor Or caged nucleic acids. is unsuitable for precise control of the duration of protein expression. Here, I report a photocontrollable cap (PC-cap) that can control the translation of mRNA in a reversible manner via its cis-trans photoisomerization through illumination with 370 and 430 nm light. 2-meta-Methyl-phenylazo cap (mMe-2PA-cap) in the trans form silences translation in zebrafish embryo, whereas treatment with the cis form provided a 7.1 times larger amount of translated protein compared to the trans form. Moreover, translation activated by illumination of the embryo with 370 nm light was rapidly inactivated again by subsequent illumination with 430 rim light. An application of this approach was demonstrated by photoinducing the development of double-headed zebrafish by controlling the expression period of squint protein.