Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens
Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens
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DOI：
10.1107/s1744309108036920
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发表时间：
2008-12-01
影响因子：
0.9
通讯作者：
Iwata, So
中科院分区：
文献类型：
--
作者：
Shimamura, Tatsuro;Yajima, Shunsuke;Iwata, So
来自产气微杆菌的整合膜蛋白Mhp 1转运乙内酰脲，属于核碱基：阳离子同向转运体1家族。成功地纯化和结晶了mhp 1。初始晶体使用悬滴气相扩散法获得，但衍射差。结晶条件的优化导致正交晶体的产生（空间群P2（1）2（1）2（1），晶胞参数a = 79.7，B = 101.1，c = 113.8埃）。一个完整的数据集已收集到一个单晶的分辨率为2.85埃与64 741独立的观察（94%完成）和R-合并为0.12。目前正在进行进一步的试验性分阶段方法。
The integral membrane protein Mhp1 from Microbacterium liquefaciens transports hydantoins and belongs to the nucleobase: cation symporter 1 family. Mhp1 was successfully purified and crystallized. Initial crystals were obtained using the hanging-drop vapour-diffusion method but diffracted poorly. Optimization of the crystallization conditions resulted in the generation of orthorhombic crystals (space group P2(1)2(1)2(1), unit-cell parameters a = 79.7, b = 101.1, c = 113.8 angstrom). A complete data set has been collected from a single crystal to a resolution of 2.85 angstrom with 64 741 independent observations (94% complete) and an R-merge of 0.12. Further experimental phasing methods are under way.