HIV-1 prevalence and subtype/recombinant distribution among travelers entering China from Vietnam at the HeKou port in the Yunnan province, China, between 2003 and 2012
HIV-1 prevalence and subtype/recombinant distribution among travelers entering China from Vietnam at the HeKou port in the Yunnan province, China, between 2003 and 2012
复制标题
2003年至2012年中国云南省河口口岸从越南入境中国的旅行者中HIV-1感染率及亚型/重组体分布
DOI：
10.1002/jmv.24202
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发表时间：
2015-09-01
影响因子：
12.7
通讯作者：
Xia, Xueshan
中科院分区：
文献类型：
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作者：
Wang, Yajuan;Liang, Yaobo;Xia, Xueshan
本研究旨在了解河口陆路口岸入境人员中HIV-1的流行情况及其亚型分布。2003年至2012年间，从从越南进入中国的人中随机招募了22,799人。在这一过境人群中，共有161人（0.71%）被确定为hiv -1阳性。收集140份hiv -1阳性血清样本进行RNA提取，rt -巢式PCR扩增组特异性抗原(gag)-RT，长度为2.6kb。分析DNA序列以确定HIV-1亚型/重组。我们发现循环重组型01_AE （CRF01_AE）是最常见的HIV-1亚型，占亚型83样本的49.4%(41/83)，其次是CRF08_BC（26.5%, 22/83）和CRF07_BC（7.2%, 6/83）。只有1例样本被归为c亚型，13例无法归为任何已知亚型或crf，呈现独特的重组形式（urf）。其中，鉴定出6种重组模式。它们具有不同的结构，由B、C、CRF01_AE、CRF07_BC和CRF08_BC亚型片段组成。2003年至2012年间，CRF01_AE和CRE08_BC是每年发现的最普遍的重组形式。但在过去十年中，各亚型的年度变化在这些旅行者中具有不确定的规律性。了解HIV-1亚型/重组在从越南通过该陆路口岸进入中国的个体中的分布及其随时间的变化，对于制定预防HIV跨境传播的策略至关重要。中华医学杂志，2015,37(2):557 - 557。(c) 2015 Wiley期刊公司
The aim of this study was to assess HIV-1 prevalence and the distribution of HIV-1 subtypes among travelers crossing the border at the HeKou land port. Between 2003 and 2012, 22,799 persons were randomly recruited from people entering China from Vietnam. In this crossing border population, a total of 161 (0.71%) travelers were determined as HIV-1-positive. From them, 140 HIV-1-positive serum samples were collected for RNA extraction and subsequent RT-nested PCR amplification of the group-specific antigen (gag)-RT with a length of 2.6kb. The DNA sequences were analyzed to determine the HIV-1 subtypes/recombinants. We found that the circulating recombinant form 01_AE (CRF01_AE) was the most common HIV-1 subtype, accounting for 49.4% (41/83) of the subtyped 83 samples, followed by CRF08_BC (26.5%, 22/83) and CRF07_BC (7.2%, 6/83). Only 1 sample was classified as subtype C. Thirteen cases could not be clustered into any known subtypes or CRFs and presented as unique recombinant forms (URFs). Of them, 6 recombination patterns were identified. They had distinct structures consisting of fragments of subtypes B, C, CRF01_AE, CRF07_BC and CRF08_BC. Between 2003 and 2012, CRF01_AE and CRE08_BC were shown to be the most prevalent recombinant forms identified each year. But yearly change of each subtype is uncertain regular among in these travelers during the past decade. Understanding the distribution of HIV-1 subtypes/recombinants and how it changes across time among individuals entering China from Vietnam through this land port is crucial to establish strategies for the prevention of HIV cross-border transmission. J. Med. Virol. 87:1500-1509, 2015. (c) 2015 Wiley Periodicals, Inc.