Beneficial effect of myricetin on renal functions in streptozotocin-induced diabetes
Beneficial effect of myricetin on renal functions in streptozotocin-induced diabetes
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DOI：
10.1007/s10238-011-0167-0
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发表时间：
2012-12-01
影响因子：
4.6
通讯作者：
Aslan, Mutay
中科院分区：
文献类型：
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作者：
Ozcan, Filiz;Ozmen, Asli;Aslan, Mutay
杨梅素是一种天然存在的类黄酮，已知可降低糖尿病患者的血糖水平;然而，其对肾功能的影响尚未确定。本研究探讨了杨梅素对糖尿病肾病发生的结构和功能变化的影响。将雄性白化病Wistar大鼠分为正常血糖组、糖尿病组和杨梅素治疗糖尿病组。通过腹膜内（ip）注射链脲佐菌素（50 mg/kg）诱导糖尿病，研究中包括空腹血糖（FBG）水平大于200 mg/dl的大鼠。在确诊糖尿病后16周开始给予杨梅素（6 mg/天，ip）治疗。在苏木精-伊红和Masson三色染色切片上进行光学显微镜检查，以评估杨梅素对肾脏结构变化的影响，同时测量肌酐清除率、血尿素氮（BUN）、肾脏重量、尿量和蛋白质，以评估肾功能。测定各组大鼠肾组织谷胱甘肽过氧化物酶（GPx）和黄嘌呤氧化酶（XO）的活性。杨梅素治疗显著降低肾小球硬化和减少BUN，尿量和蛋白排泄，这是深刻的糖尿病大鼠增加。在糖尿病大鼠中测量的肌酐清除率降低在杨梅素治疗后显著增加。杨梅素还恢复了改变的肾脏活动的GPx和XO，这是减少和增加糖尿病大鼠，分别。总之，杨梅素改善糖尿病大鼠肾功能改变，恢复肾脏GPx和XO的活性。所获得的数据表明，杨梅素可能是治疗糖尿病肾病的潜力。
Myricetin is a naturally occurring flavonoid that is known to decrease plasma glucose levels in diabetes; however, its influence on renal functions has not yet been determined. This study investigated the effect of myricetin on structural and functional changes occurring in diabetic nephropathy. Male Albino Wistar rats were divided into three groups: normoglycemic, diabetic and myricetin-treated diabetic. Diabetes was induced by intraperitoneal (ip) injection of streptozotocin (50 mg/kg), and rats having fasting blood glucose (FBG) levels greater than 200 mg/dl were included in the study. Treatment of myricetin (6 mg/day ip) was initiated 16 weeks after diabetes was confirmed. Light microscopy was performed on hematoxylin-eosin- and Masson's trichrome-stained sections to evaluate the effect of myricetin on structural changes in the kidney, while creatinine clearance, blood urea nitrogen (BUN), kidney weight, urine volume and protein were measured to assess kidney functions. Activities of glutathione peroxidase (GPx) and xanthine oxidase (XO) were also measured in renal tissues obtained from all experimental groups. Myricetin treatment significantly decreased glomerulosclerosis and reduced BUN, urinary volume and protein excretion, which was profoundly increased in diabetic rats. Decreased creatinine clearance measured in diabetic rats was significantly increased following myricetin treatment. Myricetin also restored altered renal activities of GPx and XO, which were decreased and increased in diabetic rats, respectively. In conclusion, myricetin improved altered renal functions and restored renal activities of GPx and XO in diabetic rats. Obtained data suggest that myricetin could be of therapeutic potential in diabetic nephropathy.