Bioactive silk protein biomaterial systems for optical devices
Bioactive silk protein biomaterial systems for optical devices
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DOI：
10.1021/bm701235f
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发表时间：
2008-04-01
影响因子：
6.2
通讯作者：
Omenetto, Fiorenzo G.
中科院分区：
文献类型：
--
作者：
Lawrence, Brian D.;Cronin-Golomb, Mark;Omenetto, Fiorenzo G.
基于丝素的生物材料系统以前已经被探索用于各种医疗和非医疗材料的需求。丝绸独特的生物物理特性为利用这一独特的纤维蛋白家族生成高度定制的结构和形态提供了选择。为了利用这些特性，我们优化了丝素蛋白的全水处理工艺，使其成为新型表面纳米蛋白质材料。我们利用对这种纳米形态的控制来优化这些丝蛋白系统的光学特性。我们展示了对表面形态的控制，最小可达125 nm，在大长度尺度上具有保真度。这种表面纳米技术使丝蛋白可以形成衍射光学元件，如衍射栅、图案生成器和透镜，这是由于通过控制β晶片的结晶度，将新型水处理方法制成光学透明材料。此外，我们在形成丝绸衍射栅的过程中加入了生物成分，如血红蛋白和酶过氧化物酶。蚕丝蛋白在水中的环境处理，与这些生物活性成分相结合，使这些夹带的分子保持活性，并为光学活性丝膜提供额外的功能和选择性。因此，生化和光学读出的组合是可行的，并在具有光谱辨别和生物功能的单个一次性/全部可降解元件中提供。这些新的表面纳米丝蛋白材料系统提供了独特的特征组合，可能有助于一系列生物传感器的需求，特别是当考虑到这些蛋白质的非凡机械性能、它们的生物兼容性和可控制的生物降解时。
Silk-based biomaterial systems have been previously explored for a variety of medical and nonmedical materials needs. The unique biophysical features of silks provide options to generate highly tailored structures and morphologies with this unique family of fibrous proteins. To exploit these features, we have optimized the all aqueous processing of silk fibroin into novel surface nanopatterned protein materials. We have exploited control of this nanomorphology to optimize the optical features of these silk protein systems. We demonstrate control of surface morphology down to 125 nm, with fidelity over large length scales. This surface nanopatterning allows the silk protein to be formed into diffractive optics such as diffraction gratings, pattern generators, and lenses due to novel aqueous processing into optically clear materials via control of beta sheet crystallinity. Further, we incorporate biological components, such as hemoglobin and the enzyme peroxidase, during the process of forming the silk diffraction gratings. The ambient processing of the silk protein in water, in combination with these bioactive components, allows these entrained molecules to retain activity and provide added functions and selectivity to the optically active silk films. Thus, combinations of biochemical and optical readout is feasible and provides in a single, disposable/all degradable element with both spectral discrimination and biological function. These new surface nanopatterned, bioactive silk protein-based material systems offer a unique combination of features potentially useful for a range of biosensor needs, particularly when considered in concert with the remarkable mechanical properties of these proteins, their biocompatibility, and controllable biodegradation.