Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function
Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function
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DOI：
10.1073/pnas.101120898
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发表时间：
2001-05-08
影响因子：
11.1
通讯作者：
Van De Water, TR
中科院分区：
文献类型：
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作者：
Kopke, RD;Jackson, RL;Van De Water, TR
成年豚鼠的前庭用庆大霉素损伤，然后用两种生长因子混合物（即GF I或GF II）中的任一种进行外淋巴灌注治疗。GF I含有转化生长因子α（TGF -α）、胰岛素样生长因子1（IGF - I）和视黄酸（RA），而GF II含有这三种因子以及脑源性神经营养因子。用GF I治疗显著促进了耳毒素损伤的椭圆囊中前庭毛细胞的更新以及静纤毛束形态的成熟。在GF II灌注混合物中添加脑源性神经营养因子导致耳毒素损伤的嵴中1型前庭毛细胞恢复，并改善了前庭功能。这些结果表明，生长因子疗法可能是治疗因毛细胞功能障碍和/或缺失导致的平衡失调的一种有效方法。
The vestibules of adult guinea pigs were lesioned with gentamicin and then treated with perilymphatic infusion of either of two growth factor mixtures (i.e., GF I or CF II). GF I contained transforming growth factor alpha (TGF alpha), insulin-like growth factor type one (IGF-I), and retinoic: acid (RA), whereas CF II contained those three factors and brain-derived neurotrophic: factor. Treatment with GF I significantly enhanced vestibular hair cell renewal in ototoxin-damaged utricles and the maturation of stereociliary bundle morphology. The addition of brain-derived neurotrophic factor to the GF II infusion mixture resulted in the return of type 1 vestibular hair cells in ototoxin-damaged cristae, and improved vestibular function. These results suggest that growth factor therapy may be an effective treatment for balance disorders that are the result of hair cell dysfunction and/or loss.