Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene
Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene
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DOI：
10.1038/75985
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发表时间：
2000-06-01
期刊：
影响因子：
30.8
通讯作者：
Pinkel, D
中科院分区：
文献类型：
--
作者：
Albertson, DG;Ylstra, B;Pinkel, D
我们在此表明，通过阵列比较基因组杂交1,2,3,4 （CGH）技术对扩增区域的DNA拷贝数进行定量测量，可以提供关于扩增子边界和扩增最大值位置的精确信息，从而促进癌基因鉴定。利用这种分析能力，我们在2013年的一个大约2mb的重复像差区域内解决了两个扩增区域。乳腺癌中有2个。推定的致癌基因ZNF217（参考文献5）位于一个峰上，CYP24（编码维生素D 24羟化酶）位于另一个峰上，其过表达可能导致维生素D介导的生长控制被取消（参考文献6）。
We show here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization 1, 2, 3, 4 (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima. Using this analytical capability, we resolved two regions of amplification within an approximately 2-Mb region of recurrent aberration at 20q13. 2 in breast cancer. The putative oncogene ZNF217 (ref. 5) mapped to one peak, and CYP24 (encoding vitamin D 24 hydroxylase), whose overexpression is likely to lead to abrogation of growth control mediated by vitamin D (ref. 6), mapped to the other.