Components of Adenovirus Genome Packaging.
Components of Adenovirus Genome Packaging.
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DOI：
10.3389/fmicb.2016.01503
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发表时间：
2016
影响因子：
5.2
通讯作者：
Mittal SK
中科院分区：
文献类型：
--
作者：
Ahi YS;Mittal SK
腺病毒是具有双链DNA （dsDNA）基因组的二十面体病毒。AdV中的基因组包装被认为类似于在含有二十面体噬菌体和疱疹病毒的dsDNA中看到的。AdV基因组的特异性识别是由位于病毒基因组左端附近的包装结构域介导的，并由病毒包装机械介导。近年来，我们对病毒包装机械各组成部分在AdV基因组包装中的作用的了解有了很大的进展。在没有一个或多个参与包装的成分的情况下组装的空衣壳的特征，独特顶点的鉴定，以及IVa2（假定的包装atp酶）在基因组包装中的作用的证明，提供了令人信服的证据，证明AdVs遵循顺序组装途径。本文就AdV基因组包装中涉及的各种病毒和细胞因子的功能进行了详细的讨论。最后简要讨论了空衣壳、组装中间体、支架蛋白、门脉顶点和DNA封装酶在AdV组装和包装中的作用。
Adenoviruses (AdVs) are icosahedral viruses with double-stranded DNA (dsDNA) genomes. Genome packaging in AdV is thought to be similar to that seen in dsDNA containing icosahedral bacteriophages and herpesviruses. Specific recognition of the AdV genome is mediated by a packaging domain located close to the left end of the viral genome and is mediated by the viral packaging machinery. Our understanding of the role of various components of the viral packaging machinery in AdV genome packaging has greatly advanced in recent years. Characterization of empty capsids assembled in the absence of one or more components involved in packaging, identification of the unique vertex, and demonstration of the role of IVa2, the putative packaging ATPase, in genome packaging have provided compelling evidence that AdVs follow a sequential assembly pathway. This review provides a detailed discussion on the functions of the various viral and cellular factors involved in AdV genome packaging. We conclude by briefly discussing the roles of the empty capsids, assembly intermediates, scaffolding proteins, portal vertex and DNA encapsidating enzymes in AdV assembly and packaging.