Steroidogenic enzymes, their related transcription factors and nuclear receptors in human sebaceous glands under normal and pathological conditions
Steroidogenic enzymes, their related transcription factors and nuclear receptors in human sebaceous glands under normal and pathological conditions
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DOI：
10.1016/j.jsbmb.2014.07.010
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发表时间：
2014-10-01
影响因子：
4.1
通讯作者：
Sasano, Hironobu
中科院分区：
文献类型：
--
作者：
Azmahani, Abdullah;Nakamura, Yasuhiro;Sasano, Hironobu
皮脂腺是人体皮肤中类固醇合成的主要场所，但有关正常和病理条件下人体皮脂腺中类固醇生成酶的状态及其调节的详细信息很少报道。因此，在本研究中，我们检查了正常和病理条件下人类皮脂腺中类固醇生成酶、性类固醇受体和转录因子的状态，以探讨它们在人类皮肤原位类固醇生成中的可能作用。对59例人体皮肤标本进行免疫组织化学分析，其中正常人皮脂腺22例，皮脂腺痣12例，皮脂腺增生12例，皮脂瘤3例，皮脂癌10例。用毛喉素或载体处理永生化人 SZ95 皮脂细胞 3 小时、6 小时、12 小时或 24 小时，并使用定量 RT-PCR (qPCR) 在每个时间点评估类固醇生成酶的 mRNA 水平。免疫组化结果显示，3β-HSD1、CYP11A1、StAR、17β-HSD5、CYP17A1、5α-red1、PRB、AR和NGFI-B在正常人皮脂腺中具有免疫反应性，在病理性皮脂腺中表达水平较低。体外研究结果还表明，毛喉素可提高 3β-HSD1、CYP11A1、StAR、5α-red1 和 NGFI-B 的表达水平。 3 β-HSD1 和其他类固醇生成酶在皮脂腺中表达，导致原位雄激素和孕激素合成及其功能。 (C) 2014 Elsevier Ltd. 保留所有权利。
The sebaceous gland is a major site of steroid synthesis in human skin, but details of the status of steroidogenic enzymes and their regulation in human sebaceous glands under normal and pathological conditions have rarely been reported. Therefore, in this study, we examined the status of steroidogenic enzymes, sex steroid receptors and transcription factors in human sebaceous glands under normal and pathological conditions to explore their possible roles in in situ steroid production in human skin. Immunohistochemical analysis was performed in a total of 59 human skin specimens, including 22 normal human sebaceous glands, 12 with sebaceous nevus, 12 with sebaceous gland hyperplasia, 3 with sebaceoma and 10 with sebaceous carcinoma. Immortalised human SZ95 sebocytes were treated with forskolin or vehicle for 3 h, 6 h, 12 h or 24 h, and the mRNA levels of steroidogenic enzymes were evaluated at each time point using quantitative RT-PCR (qPCR). The results of immunohistochemistry demonstrated the immunoreactivity of 3 beta-HSD1, CYP11A1, StAR, 17 beta-HSD5, CYP17A1, 5 alpha-red1, PRB, AR and NGFI-B in normal human sebaceous gland, with lower levels of expression in pathological sebaceous glands. The results of the in vitro study also indicated that the expression levels of 3 beta-HSD1, CYP11A1, StAR, 5 alpha-red1 and NGFI-B were elevated by forskolin. 3 beta-HSD1 and other steroidogenic enzymes were expressed in sebaceous glands resulting in in situ androgen and progesterone synthesis and their functions. (C) 2014 Elsevier Ltd. All rights reserved.