Genome-wide identification, evolution and expression analysis of NAC gene family under salt stress in wild emmer wheat (Triticum dicoccoides. L)
Genome-wide identification, evolution and expression analysis of NAC gene family under salt stress in wild emmer wheat (Triticum dicoccoides. L)
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DOI：
10.1016/j.ijbiomac.2023.123376
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发表时间：
2023-01-28
影响因子：
8.2
通讯作者：
Nie, Xiaojun
中科院分区：
文献类型：
--
作者：
Rui, Zesheng;Pan, Wenqiu;Nie, Xiaojun
NAC转录因子(Tf)家族是植物特有基因家族中最大的家族之一，在植物生长发育和逆境反应中发挥着重要作用。虽然NAC家族在许多植物中具有广泛的特性，但到目前为止，人们对其在野生二粒小麦中的意义还不是很清楚。本研究通过基因组搜索的方法在野生二聚体(TdNACs)中鉴定了200个NAC转录因子，根据系统发育关系将其分为12个亚家族。亚家族成员具有相似的外显子-内含子结构和相反的结构域结构。共线性分析表明，节段性复制和多倍化是TdNAC扩增的主要原因。此外，利用重新测序的数据分析了TdNacs的遗传变异，发现野生二聚体驯化栽培二粒小麦后，Nac基因出现了遗传瓶颈。最后，利用耐盐品种在盐胁迫下的RNA-SEQ数据研究了这些TdNAC的表达模式，获得了盐响应的TdNAC，其中10个TdNAC进一步用QPCR分析进行了验证。本研究为进一步研究TdNAC基因的功能提供了靶标，也为挖掘小麦等作物耐盐性改良的新基因做出了贡献。
The NAC transcription factor (TF) family is one of the largest plant-specific gene families, playing the vital roles in plant growth and development as well as stress response. Although it has been extensively characterized in many plants, the significance of NAC family in wild emmer wheat is not well understood up to now. Here, a total of 200 NAC transcription factors were identified in wild emmer (TdNACs) through a genome-search method, which were classified into 12 subfamilies based on phylogenetic relationship. And the members in the subfamily shared similar exon-intron structure and conversed domain organization. Collinearity analysis revealed that segmental duplication and polyploidization contributed mainly to the expansion of TdNACs. Furthermore, the genetic variations of TdNACs were investigated using the re-sequencing data and genetic bottleneck has occurred on NAC genes when wild emmer domesticated to cultivated emmer wheat. Finally, the expression patterns of these TdNACs were investigated using RNA-seq data of the salt-tolerant genotype under salt stress to obtain salt-responsive TdNACs, and 10 out of which were further validated using QPCR analysis. This study provided the targets for further functional study of TdNAC genes, and also contributed to mine novel genes for improving the salt tolerance in wheat and other crops.