Compartmentalization of Hepatitis C Virus Genotypes between Plasma and Peripheral Blood Mononuclear Cells
Compartmentalization of Hepatitis C Virus Genotypes between Plasma and Peripheral Blood Mononuclear Cells
复制标题
血浆和外周血单核细胞之间丙型肝炎病毒基因型的划分
作者：
A. Roque;D. Ducoulombier;G. Di Liberto;R. Kara;M. Gigou;E. Dussaix;D. Samuel;C. Féray
丙型肝炎病毒（HCV）高度保守的5 '非翻译区（UTR）变异在血浆和外周血单个核细胞（PBMC）之间存在差异。这种划分的普遍性和机制尚不清楚。采用单链构象多态性（SSCP）、克隆或系探针法（LiPA）对116例慢性感染患者（包括44例肝移植受者）的血浆和PBMC HCV变异进行比较。在可分析的109例患者中，43/109例（39%）的SSCP模式在室间存在差异。移植患者（21/38[55%]比22/71 [31%]，P < 0.01）、1991年以前通过多次输血获得HCV的患者（15/20;75%）或通过药物注射获得HCV的患者（16/31;52%）的差异明显高于通过未知途径感染HCV的患者（7/29;24%）或通过单次输血感染HCV的患者（5/29;17%,P < 0.001）。克隆5 ' UTR、LiPA分析和非结构区5B测序显示10例（9%）患者的两个室存在不同的基因型。在9例患者中，PBMC中检测到的基因型在血浆中未检测到，3例在肝脏中检测到的基因型较弱或未检测到。这种基因型区隔化在3例患者和2例肝移植后持续了数年。目前的研究表明，相当大比例的hcv感染者在他们的PBMC中有高度分化的变体，这可能是通过重复感染获得的。
ABSTRACT Differences in hepatitis C virus (HCV) variants of the highly conserved 5′ untranslated region (UTR) have been observed between plasma and peripheral blood mononuclear cells (PBMC). The prevalence and the mechanisms of this compartmentalization are unknown. Plasma and PBMC HCV variants were compared by single-strand conformation polymorphism (SSCP) and by cloning or by genotyping with a line probe assay (LiPA) in 116 chronically infected patients, including 44 liver transplant recipients. SSCP patterns differed between compartments in 43/109 analyzable patients (39%). Differences were significantly more frequent in patients with transplants (21/38 [55%] versus 22/71 [31%]; P < 0.01) and in those who acquired HCV through multiple transfusions before 1991 (15/20; 75%) or through drug injection (16/31; 52%) than in those infected through an unknown route (7/29; 24%) or through a single transfusion (5/29; 17%; P < 0.001). Cloning of the 5′ UTR, LiPA analysis, and nonstructural region 5B sequencing revealed different genotypes in the two compartments from 10 patients (9%). In nine patients, the genotype detected in PBMC was not detected in plasma and was weak or undetectable in the liver in three cases. This genotypic compartmentalization persisted for years in three patients and after liver transplantation in two. The present study shows that a significant proportion of HCV-infected subjects harbor in their PBMC highly divergent variants which were likely acquired through superinfections.
影响因子：
32.4
作者：
Erickson, AL;Kimura, Y;Walker, CM
通讯作者：
Walker, CM