Relationship between expression of HSP60, urease activity, production of vacuolating toxin, and adherence activity of Helicobacter pylori.
Relationship between expression of HSP60, urease activity, production of vacuolating toxin, and adherence activity of Helicobacter pylori.
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HSP60 表达、脲酶活性、空泡毒素产生和幽门螺杆菌粘附活性之间的关系。
DOI：
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发表时间：
1998
影响因子：
6.3
通讯作者：
Shigeru Kamiya
中科院分区：
文献类型：
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作者：
H. Yamaguchi;T. Osaki;H. Taguchi;T. Hanawa;T. Yamamoto;Shigeru Kamiya
研究了幽门螺杆菌表面热休克蛋白60 （HSP60）的表达与其致病因子脲酶活性、空泡毒素的产生及对人胃癌细胞粘附的关系。脲酶活性、空泡毒素的产生与HSP60的表达没有相关性。而对人胃癌MKN45细胞的粘附活性与HSP60的表达呈显著相关（相关系数r = 0.68）。在抗hsp60抗体的抑制实验中，我们检测了幽门螺杆菌对MKN45细胞的粘附。抗hsp60抗体抑制了13株幽门螺杆菌的粘附。结果提示幽门螺杆菌HSP60可能与幽门螺杆菌粘附人胃细胞有关。
The relationship between the expression of heat shock protein 60 (HSP60) of Helicobacterpylori on the bacterial surface and its pathogenic factors of urease activity, production of vacuolating toxin, and adherence to human gastric carcinoma cells was examined. There was no correlation between urease activity, production of vacuolating toxin, and the expression of HSP60. However, there was a significant correlation between the adherence activity to human gastric carcinoma MKN45 cells and the expression of HSP60 (correlation coefficient; r = 0.68). In an inhibition assay with anti-HSP60 antibody, we examined the adhesion of H. pylori to MKN45 cells. The adherence of 3 of 13 H. pylori strains was inhibited by anti-HSP60 antibody. The results suggest that H. pylori HSP60 may be associated with the adhesion of H. pylori to human gastric cells.