Effects of biosurfactants on assays of PCB congeners in transgenic arabidopsis plants carrying a recombinant guinea pig AhR-mediated GUS reporter gene expression system
Effects of biosurfactants on assays of PCB congeners in transgenic arabidopsis plants carrying a recombinant guinea pig AhR-mediated GUS reporter gene expression system
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DOI：
10.1080/03601234.2010.515166
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Ohkawa, Hideo
中科院分区：
文献类型：
--
作者：
Shimazu, Sayuri;Ohta, Masaya;Ohkawa, Hideo
构建了携带重组豚鼠（g）芳烃受体（AhR）介导的β-葡萄糖醛酸酶（GUS）报告基因表达系统的转基因拟南芥植株，用于检测多氯联苯（PCB）同系物。转基因拟南芥XgD 2 V11 -6对PCB 126的吸收与PCB 126诱导的GUS活性之间存在相关性。此外，植物对补充的吲哚3-乙酸（IAA）表现出诱导的GUS活性。因此，GUS测定可以反映内源性和外源性AhR配体的诱导。当从植物中分离的酵母培养物中产生的生物表面活性剂MEL-B用于检测转基因拟南芥中的PCB同系物时，它们表现出明显的PCB 126剂量依赖性和毒性等效因子（TEF）依赖性GUS活性。与在土壤中培养3 d和在培养基中培养3 d相比，在含有PCB同系物的土壤中培养7 d的植物，生物表面活性剂的效果更明显。因此，推测生物表面活性剂与多氯联苯同系物形成胶束，以被动扩散的方式被植物吸收，转运到地上部，诱导GUS活性。
The transgenic Arabidopsis plants carrying a recombinant guinea pig (g) aryl hydrocarbon receptor (AhR)-mediated beta-glucuronidase (GUS) reporter gene expression system were generated for assays of polychlorinated biphenyl (PCB) congeners. The selected transgenic Arabidopsis plant XgD2V11-6 exhibited a correlation between uptake of PCB126 and PCB126-induced GUS activity. Also, the plants showed induced GUS activity towards the supplemental indole 3-acetic acid (IAA). Thus, the GUS assay may reflect induction by both endogenous and exogenous AhR ligands. When biosurfactants, MEL-B, produced in the culture of yeast isolated from plants were used for assays of PCB congeners in the transgenic Arabidopsis plants, they showed marked PCB126 dose-dependent and toxic equivalency factor (TEF) dependent GUS activities. The effects of biosurfactants were clearer when the plants were cultivated on soils containing PCB congeners for 7 days as compared with on soils for 3 days as well as in the medium for 3 days. Threfore, it was estimated that biosurfactants form micellae with PCB congeners, which are easily uptaken by the plants in a mode of passive diffusion, transport into the aerial parts and then induce GUS activity.