Insulin like growth factor I improves yak (Bos grunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2
Insulin like growth factor I improves yak (Bos grunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2
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胰岛素样生长因子 I 通过调节 Bax 和 Bcl-2 的表达来提高牦牛 (Bos grunniens) 精子活力和卵母细胞卵裂率
DOI：
10.1016/j.theriogenology.2015.05.007
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发表时间：
2015-09-15
期刊：
影响因子：
2.8
通讯作者：
Yu, Sijiu
中科院分区：
文献类型：
--
作者：
Pan, Yangyang;Cui, Yan;Yu, Sijiu
本研究旨在探讨胰岛素样生长因子-1(IGF-1)在牦牛精子体外获能过程中对精子活力的影响，并探讨IGF-1对牦牛精子活力的影响与细胞凋亡的关系。冷冻解冻后的牦牛精子在含不同浓度(0、50、100和200 ng/mLIGF-1)的台氏碳酸氢盐缓冲培养液(Sp-talp)中38℃孵育1小时。在每一次治疗中，通过计算机辅助精子分析仪系统测量精子活动率。根据受精后卵母细胞卵裂率评价精子的受精能力。采用实时定量聚合酶链式反应和蛋白质印迹检测Bax和Bcl2的表达。值得注意的是，IGF-1提高了牦牛精子的活力和卵裂率；这些改善在100 ng/mLIGF-1组最高，其次是200 ng/mL组和50 ng/mL组，而不加IGF-1的组活力和卵裂率的改善最低。IGF-1下调Box的表达，上调Bcl2的表达。100 ng/mLIGF-1组信使RNA和Box蛋白表达最低，而Bcl2表达最高。有IGF-1组的Box表达低于无IGF-1组，有IGF-1组的Bcl2表达高于无IGF-1组。综上所述，本研究报告了添加IGF-I后牦牛精子活力和卵裂率的提高，可能是通过调节Bax和Bcl-2的表达来降低精子凋亡率的结果。(C)2015 Elsevier Inc.保留所有权利。
The aim of our present study was to examine the effects of insulinlike growth factor 1 (IGF-1) on yak sperm motility during in vitro capacitation and the relationship between the effects of IGF-1 on yak sperm motility and apoptosis was evaluated. Frozen-thawed yak spermatozoa were incubated at 38 degrees C for 1 hour in Tyrode's bicarbonate-buffered medium for sperm culture (Sp-TALP) with different concentrations (0, 50, 100, and 200 ng/mL) of IGF-1. In every treatment, the sperm motility was measured by a computer-assisted sperm analyzer system. The fertilizing ability of spermatozoa was evaluated on the basis of oocyte cleavage rate after insemination. The expression of Bax and Bcl-2 was examined by real-time polymerase chain reaction and Western blot for the messenger RNA and protein levels. It is interesting to note that IGF-1 improved yak spermatozoa motility and the cleavage rate of oocytes; these improvements were highest in the 100 ng/mL IGF-1 group, followed by the 200 ng/mL and 50 ng/mL groups, with the lowest improvements in motility and cleavage rates in groups without IGF-1. The expression level of Box was downregulated by IGF-1, whereas Bcl-2 was upregulated. Both messenger RNA and Box proteins were lowest in groups with 100 ng/mL IGF-1, where the Bcl-2 was the highest. Box expression in the groups with IGF-1 was lower than that in the group without IGF-1, and Bcl-2 expression was higher in groups with IGF-1 than that in the group without IGF-1. In conclusion, this research reports that improvements in yak spermatozoa motility and the oocyte cleavage rate after the addition of IGF-I may be a result of the reduction of spermatozoa apoptosis rates by modulating the expression of Bax and Bcl-2. (C) 2015 Elsevier Inc. All rights reserved.