Emergence of new forms of human immunodeficiency virus type 1 intersubtype recombinants in central myanmar
Emergence of new forms of human immunodeficiency virus type 1 intersubtype recombinants in central myanmar
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DOI：
10.1089/08892220050195793
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发表时间：
2000-11-01
影响因子：
1.5
通讯作者：
Takebe, Y
中科院分区：
文献类型：
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作者：
Motomura, K;Kusagawa, S;Takebe, Y
我们之前已经证明，HIV-1 env亚型B‘(B亚型中的泰国-B聚集性)和E(CRF01_AE)分布在缅甸首都仰光。然而，来自该国其他地区的艾滋病毒毒株尚未确定其基因特征。在本研究中，我们确定了来自缅甸中部(曼德勒)的25例标本的env(C2/V3)和Gag(P17)亚型。系统发育分析发现C亚型5株(20%)，CRF01_AE10株(40%)，B‘亚型4株(16%)。有趣的是，其余6份标本(24%)显示Gag和env亚型不一致；3份Gag亚型B‘/env亚型C，1份Gag亚型B’/env亚型E，1份Gag亚型C/env B‘和1份Gag亚型C/env亚型E。这些不一致的标本在注射吸毒者(4/12，33%)和从事高危行为的女性商业性工作者(2/8，25%)中频繁发现。在3个样本中验证了这些HIV-1毒株的重组性质，表明存在具有不同重组断裂点的新形式的HIV-1亚型C/B‘和C/B’/E重组体。这些数据表明，B‘、C和CRF01_AE的多个亚型在缅甸中部共同传播，导致在该地区艾滋病毒感染率最高的风险人群中进化出新形式的亚型间重组。
We have previously shown that HIV-1 env subtypes B' (a Thai-B cluster within subtype B) and E (CRF01_ AE) are distributed in Yangon, the capital city of Myanmar. However, HIV strains from the rest of country have not yet been genetically characterized. In the present study, we determined env (C2/V3) and gag (p17) subtypes of 25 specimens from central Myanmar (Mandalay). Phylogenetic analyses identified 5 subtype C (20%), in addition to 10 CRF01_ AE (40%) and 4 subtype B' (16%). Interestingly, the remaining six specimens (24%) showed discordance between gag and env subtypes; three gag subtype B'/env subtype C, one gag subtype B'/env subtype E, one gag subtype C/env subtype B', and one gag subtype C/env subtype E. These discordant specimens were found frequently among injecting drug users (4 of 12, 33%) and female commercial sex workers (2 of 8, 25%) engaging in high-risk behaviors. The recombinant nature of these HIV-1 strains was verified in three specimens, indicating the presence of new forms of HIV-1 intersubtype C/B' and C/B'/E recombinants with different recombination breakpoints. The data suggest that multiple subtypes of B', C, and CRF01_ AE are cocirculating in central Myanmar, leading to the evolution of new forms of intersubtype recombinants among the risk populations exhibiting one of the highest HIV infection rates in the region.