Microscopic Imaging of Chiral Amino Acids in Agar Gel through Circularly Polarized Luminescence of EuIII Complex
Microscopic Imaging of Chiral Amino Acids in Agar Gel through Circularly Polarized Luminescence of EuIII Complex
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DOI：
10.1002/asia.201901495
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发表时间：
2019-11-28
影响因子：
4.1
通讯作者：
Iwamura, Munetaka
中科院分区：
文献类型：
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作者：
Koike, Hikaru;Nozaki, Koichi;Iwamura, Munetaka
我们最近发现，在晶体中具有非手性结构的[Eu（pda）（2）]（-）（pda：1，10-菲咯啉-2，9-二羧酸）在含有手性氨基酸如精氨酸和组氨酸的水溶液中显示出圆偏振发光（CPL）。对琼脂凝胶进行CPL测量，其包括[Eu（pda）（2）]（-）和手性精氨酸或组氨酸的水溶液。的光谱形状，浓度，和pH值对CPL强度在琼脂凝胶中的依赖性是非常接近的那些在水溶液中，这表明在琼脂凝胶中的Eu-III复合物的CPL诱导机制类似于在水溶液中。我们进行了空间分辨CPL测量使用实验室建立的显微CPL光谱系统的琼脂凝胶样品，其中d-和l-氨基酸分别分散。我们成功地记录CPL成像图显示空间分散的D-和L-氨基酸的琼脂凝胶。
We recently found that [Eu(pda)(2)](-) (pda: 1,10-phenanthroline-2,9-dicarboxylic acid), which has an achiral structure in crystals, exhibits circularly polarized luminescence (CPL) in aqueous solutions containing chiral amino acids such as arginine and histidine. CPL measurements were performed for agar gel, which includes an aqueous solution of [Eu(pda)(2)](-) and chiral arginine or histidine. The spectral shape, concentration, and pH dependences on CPL intensity in the agar gels were very close to those in aqueous solutions, indicating that the CPL of the Eu-III complex in the agar gels was induced by mechanism similar to that in aqueous solutions. We performed spatially resolved CPL measurements using a laboratory-built microscopic CPL spectroscopic system for agar-gel samples, where d- and l- amino acids were separately dispersed. We successfully recorded CPL imaging maps showing spatial dispersions of d- and l-amino acid in the agar gels.