Royal jelly has estrogenic effects in vitro and in vivo
Royal jelly has estrogenic effects in vitro and in vivo
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DOI：
10.1016/j.jep.2005.04.012
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发表时间：
2005-10-03
影响因子：
5.4
通讯作者：
Miyata, T
中科院分区：
文献类型：
--
作者：
Mishima, S;Suzuki, KM;Miyata, T
传统上认为，来自蜜蜂 (Apis mellifera) 的蜂王浆 (RJ) 可以改善更年期症状。使用各种方法研究了 RJ 的潜在雌激素活性。 RJ 竞争 17β-雌二醇与人类雌激素受体 a 和 0 的结合，但与己烯雌酚和植物雌激素相比，其亲和力较弱。报告基因表达分析表明，0.1-1 mg/ml RJ 激活雌激素受体，通过雌激素反应元件增强报告基因的转录。 1 mg/ml RJ 通过增加 MCF-7 细胞中的基因转录来刺激雌激素反应性 pS2 和血管内皮生长因子 (VEGF) 的 mRNA 表达。用浓度范围为 0.5 至 1 mg/ml 的 RJ 处理可增强 MCF-7 细胞增殖，但同时用 1 μM 他莫昔芬处理可阻断这种作用。使用卵巢切除大鼠进行的体内研究表明，17β-雌二醇（20 mg/kg，皮下注射）治疗可恢复子宫和大脑中的 VEGF 表达，而 RJ（1 g/kg，皮下注射）可恢复子宫中的 VEGF 表达，但不能恢复大脑中的 VEGF 表达。这些发现证明 RJ 通过与雌激素受体相互作用以及内源基因表达而具有雌激素活性。 (C) 2005 Elsevier Ireland Ltd. 保留所有权利。
Royal jelly (RJ) from honeybees (Apis mellifera) is traditionally thought to improve menopausal symptoms. The potential estrogenic activities of RJ were investigated using various approaches. RJ competed for binding of 17 beta-estradiol to the human estrogen receptor a and 0 but its affinities were weak compared with diethylstilbestrol and phytoestrogens. The reporter gene expression assays suggested that 0.1-1 mg/ml RJ activated estrogen receptors, leading to enhanced transcription of a reporter gene through an estrogen-responsive element. 1 mg/ml RJ stimulated the mRNA expression of estrogen-responsive pS2 and vascular endothelial growth factor (VEGF) by increasing gene transcription in MCF-7 cells. Treatment with RJ at concentrations ranging from 0.5 to 1 mg/ml enhanced MCF-7 cell proliferation, but concomitant treatment with 1 mu M tamoxifen blocked this effect. In vivo studies using ovariectomized rats showed that 17 beta-estradiol (20 mg/kg, s.c.) treatment restored VEGF expression in both uterus and brain, whereas RJ (1 g/kg, s.c.) restored it in uterus but not in brain. These findings provide evidence that RJ has estrogenic activities through interaction with estrogen receptors followed by endogenous gene expressions. (C) 2005 Elsevier Ireland Ltd. All rights reserved.