A Genome-Wide Analysis of the Penumbral Volume in Inbred Mice following Middle Cerebral Artery Occlusion.
A Genome-Wide Analysis of the Penumbral Volume in Inbred Mice following Middle Cerebral Artery Occlusion.
复制标题
大脑中动脉闭塞后近交小鼠半影体积的全基因组分析。
DOI：
10.1038/s41598-019-41592-5
复制
发表时间：
2019
影响因子：
4.6
通讯作者：
Du,Rose
中科院分区：
文献类型：
--
作者：
Rudy,RobertF;Charoenvimolphan,Nareerat;Qian,Baogang;Berndt,Annerose;Friedlander,RobertM;Weiss,ScottT;Du,Rose
Following ischemic stroke, the penumbra, at-risk neural tissue surrounding the core infarct, survives for a variable period of time before progressing to infarction. We investigated genetic determinants of the size of penumbra in mice subjected to middle cerebral artery occlusion (MCAO) using a genome-wide approach. 449 male mice from 33 inbred strains underwent MCAO for 6 hours (215 mice) or 24 hours (234 mice). A genome-wide association study using genetic data from the Mouse HapMap project was performed to examine the effects of genetic variants on the penumbra ratio, defined as the ratio of the infarct volume after 6 hours to the infarct volume after 24 hours of MCAO. Efficient mixed model analysis was used to account for strain interrelatedness. Penumbra ratio differed significantly by strain (F = 2.7, P < 0.001) and was associated with 18 significant SNPs, including 6 protein coding genes. We have identified 6 candidate genes for penumbra ratio:Clint1,Nbea,Smtnl2,Rin3,Dclk1, andSlc24a4.
