Comparison of methods to assess the enzyme accessibility and hydrolysis of pretreated lignocellulosic substrates
Comparison of methods to assess the enzyme accessibility and hydrolysis of pretreated lignocellulosic substrates
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DOI：
10.1007/s10529-009-9993-5
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发表时间：
2009-08-01
影响因子：
2.7
通讯作者：
Saddler, Jack N.
中科院分区：
文献类型：
--
作者：
Chandra, Richard P.;Ewanick, Shannon M.;Saddler, Jack N.
评估了纤维尺寸分析、保水值和西蒙斯染色测量预测给定底物对酶水解的敏感性的潜力。对纤维尺寸分析和保水方案进行了轻微修改，以适应这些测量以评估纤维素水解的基材而不是造纸纸浆。黑松在不同条件下通过蒸汽和乙醇-有机溶剂工艺进行预处理。西蒙斯染色程序被证明是一种有效的方法，用于指示通过任一工艺预处理或改变预处理条件时底物酶促水解的潜在容易程度。
Fiber size analysis, water retention value, and Simons' stain measurements were assessed for their potential to predict the susceptibility of a given substrate to enzymatic hydrolysis. Slight modifications were made to the fiber size analysis and water retention protocols to adapt these measurements to evaluate substrates for cellulolytic hydrolysis rather than pulps for papermaking. Lodgepole pine was pretreated by the steam and ethanol-organosolv processes under varying conditions. The Simons' stain procedure proved to be an effective method for indicating the potential ease of enzymatic hydrolysis of substrates pretreated by either process or when the pretreatment conditions were altered.