Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells.
Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells.
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DOI：
10.4049/jimmunol.158.3.1108
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发表时间：
1997-02
影响因子：
4.4
通讯作者：
A. Oliver;Flavius Martin;John F. Kearney
中科院分区：
文献类型：
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作者：
A. Oliver;Flavius Martin;John F. Kearney
生发中心的形成是 T 细胞丰富区域中 B 细胞抗原刺激的结果。 B 细胞在生发中心循环，一小部分存活下来成为浆细胞或记忆 B 细胞。从成熟 B 细胞向生发中心 B 细胞并最终向终末分化 B 细胞的转化尚不清楚。人 CD38 在生发中心 B 细胞和浆细胞上高度表达，可用于描述这些 B 细胞亚群以及了解这些 B 细胞发育中涉及的信号转导事件。为了确定活化的生发中心 B 细胞和生发中心 B 细胞上的 CD38 表达是否影响生发中心分化，我们研究了小鼠中 CD38 的表达。 CD38 在派尔氏淋巴结中的滤泡 B 细胞上表达，但在位于派尔氏淋巴结内的生发中心 B 细胞上表达下调。 CD38dim/-B220+ 生发中心 B 细胞也在免疫小鼠的脾脏中发现，但在对照小鼠中未发现，这表明 Ag 刺激的生发中心形成参与了 CD38dim/-B220+ B 细胞的产生。此外，从体外 LPS 培养物中分离出的成熟浆细胞不表达 CD38，但含有高水平的细胞质 Ig。这些结果与人类研究形成鲜明对比，在人类研究中，滤泡 B 细胞上没有发现 CD38，但在生发中心 B 细胞和浆细胞上高度表达。
Germinal center formation is the result of antigenic stimulation of B cells in a T cell-rich area. B cells cycle through the germinal centers, and a small percentage survive to become plasma cells or memory B cells. The transformation from a mature B cell into a germinal center B cell and finally into a terminally differentiated B cell is not well understood. Human CD38 is highly expressed on both germinal center B cells and plasma cells, and is useful in delineating these B cell subsets and in understanding the signaling events involved in the development of these B cells. To determine whether CD38 expression on activated germinal center B cells and postgerminal center B cells influences germinal center differentiation, we studied the expression of CD38 in the mouse. CD38 is expressed on follicular B cells in the Peyer's patches but is down-regulated on germinal center B cells located within the Peyer's patches. CD38dim/-B220+ germinal center B cells are also found in the spleens of immunized but not control mice, suggesting that Ag-stimulated germinal center formation is involved in the production of CD38dim/-B220+ B cells. Furthermore, mature plasma cells isolated from in vitro LPS cultures do not express CD38, but do contain high levels of cytoplasmic Ig. These results are in contrast to studies in humans in which CD38 is not found on follicular B cells but is highly expressed on germinal center B cells and plasma cells.