INTEGRATIVE REPAIR OF ARTICULAR-CARTILAGE IN-VITRO - ADHESIVE STRENGTH OF THE INTERFACE REGION
INTEGRATIVE REPAIR OF ARTICULAR-CARTILAGE IN-VITRO - ADHESIVE STRENGTH OF THE INTERFACE REGION
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DOI：
10.1002/jor.1100130515
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发表时间：
1995-09-01
影响因子：
2.8
通讯作者：
SAH, RL
中科院分区：
文献类型：
--
作者：
REINDEL, ES;AYROSO, AM;SAH, RL
本研究的目的是量化在体外培养系统中维持并列的软骨外植体对之间的界面形成的修复组织的强度。从小牛和成年牛身上采集关节软骨外植体，解剖成均匀的块，并在保持4 x 5 mm组织重叠面积的腔室中成对孵育。在1-3周的孵育后，通过测试拉伸单搭结构的样品来评估综合修复，以估计粘合强度。在含20%胎牛血清的培养基中培养后，犊牛软骨块对和成年牛软骨块对之间的黏附强度分别以7.0和10.5 kPa/周的速度增加。这种修复过程似乎依赖于活细胞，因为成年牛软骨在孵育前的冻干完全抑制了具有可测量粘附强度的界面的发展。修复过程依赖于培养基中的血清成分。样品对在添加20%胎牛血清的培养基中孵育3周，蛋白多糖含量相对较高，黏附强度相对较高（34 kPa），而在添加或不添加0.1%牛血清白蛋白的基础培养基中孵育，蛋白多糖含量降低54-70%，黏附强度降低65-88%。在血清中培养3周的样品比在基础培养基中培养的样品DNA含量高20%。组织学分析表明，在外植体的自由表面有细胞分裂，外植体之间的界面区域也有细胞分裂。
The objective of this study was to quantify the strength of the repair tissue that forms at the interface between pairs of cartilage explants maintained in apposition in an in vitro culture system. Articular cartilage explants were harvested from calves and from adult bovine animals, dissected into uniform blocks, and incubated in pairs within a chamber that maintained a 4 x 5 mm area of tissue overlap. Following 1-3 weeks of incubation, integrative repair was assessed by testing samples in a tensile single-lap configuration to estimate adhesive strength. After incubation in medium containing 20% fetal bovine serum, the adhesive strength between pairs of calf cartilage blocks and pairs of adult bovine cartilage blocks increased at a rate of 7.0 and 10.5 kPa/week, respectively. This repair process appeared to be dependent on viable cells, since lyophilization of adult bovine cartilage before incubation completely inhibited the development of an interface with a measurable adhesive strength. The repair process was dependent on serum components in the medium. Incubation of sample pairs for 3 weeks in medium supplemented with 20% fetal bovine serum resulted in a relatively high proteoglycan content as well as a relatively high adhesive strength (34 kPa), whereas incubation in basal medium with or without 0.1% bovine serum albumin resulted in a 54-70% lower proteoglycan content and a 65-88% lower adhesive strength. Samples incubated for 3 weeks with serum also had a 20% higher DNA content than samples maintained in basal medium. Histological analysis indicated some cell division at the free surfaces of the explant and also occasional cells within the interface region between explants.