Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing.
Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing.
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DOI：
10.1016/j.fsi.2016.03.159
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发表时间：
2016-07
影响因子：
4.7
通讯作者：
Bei Wang;Z. Gan;S. Cai;Zhongliang Wang;Dapeng Yu;Ziwei Lin;Yishan Lu;Zao-he Wu;J. Jian
中科院分区：
文献类型：
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作者：
Bei Wang;Z. Gan;S. Cai;Zhongliang Wang;Dapeng Yu;Ziwei Lin;Yishan Lu;Zao-he Wu;J. Jian
MicroRNA是一类参与多种生物过程的小型非编码RNA。 microRNA 表达失调与多种疾病有关。罗非鱼（Oreochromis niloticus）是我国重要的经济鱼类。鉴定 miRNA 并研究 O 的免疫相关 miRNA。 尼罗罗非鱼中，我们应用高通量测序技术来鉴定和分析感染无乳链球菌的罗非鱼的 miRNA，时间为 72 小时，分为六个不同的时间点。结果显示，共鉴定出3009个罗非鱼miRNA，其中1121个在现有数据库中具有同源性的miRNA和1878个新的miRNA。 218 个罗非鱼 miRNA 的表达水平在细菌感染后 6 h-72 h (pi) 期间显着改变，因此这些 miRNA 被归类为差异表达的罗非鱼 miRNA。对于 1121 个差异表达的罗非鱼 miRNA，靶向 41961 个基因。 GO和KEGG富集分析显示，罗非鱼miRNA的一些靶基因主要分为凋亡过程、信号通路和免疫应答等类别。这是首次对O进行全面鉴定的报告。 在正常条件下，与尼罗罗非鱼相关的 miRNA 在脾脏中受到差异性调节。 无乳感染。这项工作为进一步了解 miRNA 调控的分子机制提供了机会。 尼罗罗非鱼宿主-病原体相互作用。
MicroRNAs are a kind of small non-coding RNAs that participate in various biological processes. Deregulated microRNA expression is associated with several types of diseases. Tilapia (Oreochromis niloticus) is an important commercial fish species in China. To identify miRNAs and investigate immune-related miRNAs ofO. niloticus, we applied high-throughput sequencing technology to identify and analyze miRNAs from tilapia infected withStreptococcus agalactiaeat a timescale of 72 h divided into six different time points. The results showed that a total of 3009 tilapia miRNAs were identified, including in 1121 miRNAs which have homologues in the currently available databases and 1878 novel miRNAs. The expression levels of 218 tilapia miRNAs were significantly altered at 6 h–72 h post-bacterial infection (pi), and these miRNAs were therefore classified as differentially expressed tilapia miRNAs. For the 1121 differentially expressed tilapia miRNAs target 41961 genes. GO and KEGG enrichment analysis revealed that some target genes of tilapia miRNAs were grouped mainly into the categories of apoptotic process, signal pathway, and immune response. This is the first report of comprehensive identification ofO. niloticusmiRNAs being differentially regulated in spleen in normal conditions relating toS. agalactiaeinfection. This work provides an opportunity for further understanding of the molecular mechanisms of miRNA regulation inO. niloticushost-pathogen interactions.