Maturation of the nodule-specific transcript MsHSF1c in Medicago sativa may involve interallelic trans-splicing
Maturation of the nodule-specific transcript MsHSF1c in Medicago sativa may involve interallelic trans-splicing
复制标题
紫花苜蓿根瘤特异性转录本 MsHSF1c 的成熟可能涉及等位基因间反式剪接
DOI：
10.1016/j.ygeno.2008.04.007
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发表时间：
2008-08-01
期刊：
影响因子：
4.4
通讯作者：
Yu, Guan-qiao
中科院分区：
文献类型：
--
作者：
He, Zhi-shui;Zou, Hua-song;Yu, Guan-qiao
在非植物物种中，许多热休克转录因子（hsf）经历了时空特异性的选择性剪接。然而，对植物中hsf的时空特异性剪接知之甚少。此前，我们报道了苜蓿HSF基因MsHSF1在不同组织中经历了多个选择性剪接事件。在这里，我们鉴定了另一个剪接转录异构体MsHSF1c，包含177个碱基的串联重复，并表明低丰度的MsHSF1c是MsHSF1的结节特异性转录物。我们还发现MsHSF1存在多个单碱基变异等位基因，并且MsHSF1等位基因的表达在苜蓿根瘤中存在等位基因特异性差异。由于1006位点的单碱基变异将MsHSF1b的at改变为MsHSF1b-3的GT，从而与MsHSF1b/1b-1在827-828位点的AG形成一对供体/受体位点，用于mrna前剪接，我们认为MsHSF1c可能是由等位基因MsHSF1b-3和MsHSF1b或MsHSF1b-1之间的反式剪接产生的。这些结果为植物HSF基因转录中组织特异性贡献的作用提供了新的见解。2008爱思唯尔公司版权所有。
In nonplant species, many heat-shock transcription factors (HSFs) undergo spatiotemporal-specific alternative splicing. However, little is known about the spatiotemporal-specific splicing of HSFs in plants. Previously, we reported that the alfalfa HSF gene MsHSF1 undergoes multiple alternative splicing events in various tissues. Here, we identified another spliced transcript isoform, MsHSF1c, containing a 177-base tandem repeat, and showed that the low-abundance MsHSF1c is a nodule-specific transcript of MsHSF1. We also found that MsHSF1 presents multiple alleles with single-base variations and the expression of MsHSF1 alleles has allele-specific differences in alfalfa nodules. Because single-base variations at position 1006 change the AT of MsHSF1b to GT in MsHSF1b-3, creating a pair of donor/acceptor sites with the AG of MsHSF1b/1b-1 at position 827-828 for pre-mRNA splicing, we suggest that MsHSF1c may be generated by trans-splicing between alleles MsHSF1b-3 and MsHSF1b or MsHSF1b-1. These results provide new insight into the role of tissue-specific contribution in the transcription of plant HSF genes. 0 2008 Elsevier Inc. All rights reserved.