Primary structure of a calcium channel that is highly expressed in the rat cerebellum.
Primary structure of a calcium channel that is highly expressed in the rat cerebellum.
复制标题
在大鼠小脑中高度表达的钙通道的一级结构。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
T. Snutch
中科院分区：
文献类型：
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作者：
T. Starr;W. Prystay;T. Snutch
以往的分子克隆实验表明，哺乳动物脑中表达电压门控性钙通道α1亚基的多种亚型(分别命名为RBa、RBB、RBC和RBD)。我们报道了编码大鼠脑A类钙通道的cDNAs的分离和鉴定。该基因编码2212个氨基酸，与心肌和骨骼肌二氢吡啶敏感钙通道的α1亚基有33%的序列同源性。与其他钙通道相比，RBA通道在羧基末端和结构域II和III之间的大亲水性片段(474个氨基酸)上都有显著不同。Northern印迹分析表明，RBA转录本在大鼠中枢神经系统的所有区域都有表达，但以小脑最为显著。PCR分析表明，在大鼠的心脏和垂体中存在A类转录本，而在脾、肾或肝脏中不存在A类转录本。该基因编码钙通道α1亚基，不同于二氢吡啶敏感的钙通道序列，在多种可兴奋细胞中均有表达。
Previous molecular cloning experiments showed that multiple isoforms of the alpha 1 subunit of voltage-gated Ca channels are expressed in the mammalian brain (designated rbA, rbB, rbC, and rbD). We report here the isolation and characterization of cDNAs encoding the rat brain class A (rbA) Ca channel. The rbA-126 cDNA encodes a 2212-amino acid protein that shares 33% sequence identity with the alpha 1 subunits of cardiac and skeletal muscle dihydropyridine-sensitive Ca channels. When compared with other Ca channels, the rbA channel is notably different in both the carboxyl terminus and in a large (474-amino acid) hydrophilic segment between domains II and III. Northern blot analysis shows that rbA transcripts are expressed in all regions of the rat central nervous system, but most prominently in the cerebellum. A more widespread distribution of rbA Ca channels is indicated by PCR analysis, which demonstrates the presence of class A transcripts in the rat heart and pituitary but not in the spleen, kidney, or liver. The rbA cDNA appears to encode a Ca channel alpha 1 subunit that is distinct from the dihydropyridine-sensitive Ca channel sequences and that is expressed in a variety of excitable cells.