Direct stimulation by purified GM-CSF of the proliferation of multipotential and erythroid precursor cells.
Direct stimulation by purified GM-CSF of the proliferation of multipotential and erythroid precursor cells.
复制标题
纯化的 GM-CSF 直接刺激多能细胞和红系前体细胞的增殖。
作者：
D. Metcalf;G. Johnson;A. Burgess
gm - csf。从小鼠肺条件培养基中纯化。刺激粒细胞-巨噬细胞集落的形成，但在琼脂培养的12天CBA胎儿肝细胞中不能刺激任何类型的纯或混合红系集落的形成。然而。细胞与纯化的GM-CSF初始孵育2天。然后加入小鼠脾条件培养基（SCM）。允许高达50%的红细胞或混合造血菌落的形成，从一开始就受到SCM的刺激。通过沉降速度或细胞周期状态，这些gm - csf反应性集落形成细胞与其余形成红系或混合集落的细胞无法区分。302个克隆被纯化的GM-CSF刺激增殖。25个形成红细胞或混合造血菌落转移到含有SCM的培养基上。当转移到含有纯化GM-CSF的培养基中时，由SCM启动的克隆不能形成红系或混合菌落。用纯化的GM-CSF刺激12天CBA胎儿外周血的单细胞增殖，并在加入SCM后形成纯或混合红系菌落。GM-CSF因此能够直接刺激至少50%可检测到的多电位和早期红系前体细胞的多达5次细胞分裂。
GM-CSF. purified from mouse lung-conditioned medium. stimulated granulocyte-macrophage colony formation but was unable to stimulate the formation of any type of pure or mixed erythroid colony in agar cultures of 1 2-day CBA fetal liver cells. However. initial incubation of cells for 2 days with purified GM-CSF. followed by addition of mouse spleenconditioned medium (SCM). allowed the formation of up to 50% of the erythroid or mixed hemopoietic colonies expected in cultures stimulated from the outset by SCM. These GM-CSF-responsive colony-forming cells were not distinguishable from the remainder of cells forming erythroid or mixed colonies by sedimentation velocity or cell cycle status. From 302 clones stimulated to proliferate by purified GM-CSF. 25 formed erythroid or mixed hemopoietic colonies on transfer to cultures containing SCM. Clones initiated by SCM were unable to form erythroid or mixed colonies when transferred to cultures containing purified GM-CSF. Single cells from 12-day CBA fetal peripheral blood also were stimulated to proliferate by purified GM-CSF and subsequently formed pure or mixed erythroid colonies when SCM was added. GM-CSF is thus able to directly stimulate up to 5 cell divisions of at least 50% of detectable multipotential and early erythroid precursor cells.