Biofilm formation by Salmonella spp. in catfish mucus extract under industrial conditions.
Biofilm formation by Salmonella spp. in catfish mucus extract under industrial conditions.
复制标题
DOI：
10.1016/j.fm.2017.09.016
复制
发表时间：
2018-04
影响因子：
5.3
通讯作者：
Nitin Dhowlaghar;Piumi De Abrew Abeysundara;R. Nannapaneni;M. Schilling;Sam K C Chang;W. Cheng;C. S. Sharma
中科院分区：
文献类型：
--
作者：
Nitin Dhowlaghar;Piumi De Abrew Abeysundara;R. Nannapaneni;M. Schilling;Sam K C Chang;W. Cheng;C. S. Sharma
The objective of this study was to determine the effect of strain and temperature on the growth and biofilm formation ofSalmonellaspp. in high and low concentrations of catfish mucus extract on different food-contact surfaces at 22 °C and 10 °C. The second objective of this study was to evaluate the efficacy of disinfectants at recommended concentrations and contact times for removingSalmonellabiofilms cells on a stainless steel surface containing catfish mucus extract. Growth and biofilm formation of allSalmonellastrains increased with higher concentrations of catfish mucus extract at both 10 °C and 22 °C. In 15 μg/ml of catfish mucus extract inoculated with 3 log CFU/ml, the biofilm levels ofSalmonellaon stainless steel surface reached to 3.5 log CFU/cm2at 10 °C or 5.5 log CFU/cm2at 22 °C in 7 days. In 375 μg/ml of catfish mucus extract inoculated with 3 log CFU/ml, the biofilm levels ofSalmonellaon the stainless steel surface reached 4.5 log CFU/cm2at 10 °C and 6.5 log CFU/cm2at 22 °C in 7 days. No differences were observed betweenSalmonellastrains tested for biofilm formation in catfish mucus extract on the stainless steel surface. The biofilm formation bySalmonellaBlockley (7175) in catfish mucus extract was less (P< 0.05) on buna-N rubber when compared to stainless steel, polyethylene and polyurethane surfaces.Salmonellabiofilm cells were not detectable on the stainless steel surface after treatment with a mixture of disinfectants but were still present when single compound disinfectants were used.
