Simulation of the mechanical response of cells on micropost substrates.
Simulation of the mechanical response of cells on micropost substrates.
复制标题
模拟细胞在微柱基底上的机械响应。
DOI：
10.1115/1.4025114
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
J. McGarry
中科院分区：
文献类型：
--
作者：
W. Ronan;Amit Pathak;V. Deshpande;R. McMeeking;J. McGarry
将细胞植入微柱阵列以量化其收缩行为的实验研究正变得越来越普遍。由于细胞收缩性和机械转导过程的复杂性，解释由这种实验技术产生的数据是困难的。在当前的研究中，考虑应变速率依赖的收缩性和细胞骨架重塑的耦合框架与张力依赖的焦点黏附形成的热力学模型串联使用，以研究细胞粘附在微柱阵列上的生物力学响应。提出了以下实验研究的计算研究：不同尺寸阵列上的单元行为与后刚度范围；不规则细胞内应力纤维与黏附灶的形成；单元对局部应用于单个桩的变形的响应；以及细胞对微柱阵列等双轴拉伸的响应。预测应力纤维和焦点黏附分布；此外，预测的后牵引力在定量和定性上都得到了先前发表的实验数据的支持。因此，本研究中提出的计算模型为实验微帖子研究的设计和解释提供了一个框架。
Experimental studies where cells are seeded on micropost arrays in order to quantify their contractile behavior are becoming increasingly common. Interpretation of the data generated by this experimental technique is difficult, due to the complexity of the processes underlying cellular contractility and mechanotransduction. In the current study, a coupled framework that considers strain rate dependent contractility and remodeling of the cytoskeleton is used in tandem with a thermodynamic model of tension dependent focal adhesion formation to investigate the biomechanical response of cells adhered to micropost arrays. Computational investigations of the following experimental studies are presented: cell behavior on different sized arrays with a range of post stiffness; stress fiber and focal adhesion formation in irregularly shaped cells; the response of cells to deformations applied locally to individual posts; and the response of cells to equibiaxial stretching of micropost arrays. The predicted stress fiber and focal adhesion distributions; in addition to the predicted post tractions are quantitatively and qualitatively supported by previously published experimental data. The computational models presented in this study thus provide a framework for the design and interpretation of experimental micropost studies.
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影响因子：
6.1
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通讯作者：
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影响因子：
3.4
作者：
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50.3
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.4
作者：
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通讯作者：
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