Imaging of metals and metal-containing species in biological tissues and on gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS): A new analytical strategy for applications in life sciences
Imaging of metals and metal-containing species in biological tissues and on gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS): A new analytical strategy for applications in life sciences
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DOI：
10.1351/pac200880122643
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发表时间：
2008-12-01
影响因子：
1.8
通讯作者：
Becker, J. Susanne
中科院分区：
文献类型：
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作者：
Becker, J. Sabine;Zoriy, M.;Becker, J. Susanne
激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）已成为。在生命科学中建立了一种高效、灵敏的痕量、超痕量和表面分析技术。我们开发了一种新的分析成像技术，利用LA-ICP-MS来研究生物组织中的元素分布。如今，利用双聚焦扇区场（LA-ICP-SFMS）或四极杆质谱（LA-ICP-QMS）成像LA-ICP-MS可以作为一种令人兴奋的工具，为生物系统中感兴趣的元素的病理生理学、药理学和毒理学提供新的信息。生物组织中元素（如Cu、Fe、Zn、Se等）的定量测定日益引起人们的兴趣，特别是在脑研究中（例如，用于研究神经退行性疾病，如阿尔茨海默病或帕金森病）。采用LA-ICP-SFMS在不同大小的薄组织切片（如对照人类或大鼠脑组织或肿瘤区域）中生成详细的区域特异性元素分布图像。此外，利用LA-ICP-QMS成像质谱技术研究植物组织中营养和有毒元素的吸收和转运。除了组织中必需元素和有毒元素的定量成像外，还需要强大的分析技术来测定和表征大量蛋白质中磷蛋白和含金属蛋白，并将其电泳分离[例如蓝色天然聚丙烯酰胺凝胶电泳（BN-PAGE）和十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）]成ID和2D凝胶。利用LA-ICP-MS检测ID凝胶蛋白带或2D凝胶电泳（2D- ge）分离的蛋白点中的金属蛋白。除了LA-ICP-MS进行元素测定外，还采用基质辅助激光解吸/电离（MALDI-MS）对含金属蛋白进行了鉴定。本文将讨论LA-ICP-MS在生命科学领域的最新进展，包括组织薄片成像以及结合MALDI-MS在蛋白质组学分析中的应用，以研究磷蛋白和含金属蛋白。
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has become. established as a very efficient and sensitive trace, ultratrace, and surface analytical technique in the life sciences. We have developed a new analytical imaging technique using LA-ICP-MS to study element distribution in biological tissues. Nowadays, imaging LA-ICP-MS using double-focusing sector field (LA-ICP-SFMS) or quadrupole-based mass spectrometers (LA-ICP-QMS) can be applied as an exciting tool providing new information on the pathophysiology, pharmacology, and toxicology of elements of interest in biological systems. The quantitative determination of elements (e.g., Cu, Fe, Zn, Se, and others) in biological tissues is of growing interest especially in brain research (e.g., for studying neurode-generative diseases such as Alzheimer's or Parkinson's disease). LA-ICP-SFMS was employed to produce images of detailed regionally specific element distributions in thin tissue sections of different sizes (such as control human or rat brain tissues or tumor regions). In addition, imaging MS using LA-ICP-QMS was applied to study the uptake and transport of nutrient and toxic elements in plant tissues.Besides the quantitative imaging of essential and toxic elements in tissues, powerful analytical techniques are also required for the determination and characterization of phosphoproteins and metal-containing proteins within a large pool of proteins, after electrophoretic separation [e.g., blue native polyacrylamide gel electrophoresis (BN-PAGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)] into ID and 2D gels. LA-ICP-MS was used to detect metalloproteins in protein bands of ID gels or protein spots separated after 2D gel electrophoresis (2D-GE). In addition to elemental determination by LA-ICP-MS, matrix-assisted laser desorption/ionization, or MALDI-MS, was employed to identify metal-containing proteins.Recent progress will be discussed in applying LA-ICP-MS in the life sciences, including the imaging of thin slices of tissue and applications in proteome analysis in combination with MALDI-MS to investigate phosphoproteins and metal-containing proteins.